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1. Introduction
2. The cell cycle
3. Crystal structure of Cdk2 bound to cyclin A
4. Periodicity in cyclin expression
5. Regulation of Cdks
6. Focusing on Cdk2
7. Cdk2
8. Cdk2-/- knockout mice
9. Cdk2-/- knockout mice are viable
10. Atrophy in testis of Cdk2-/- mice
11. Absence of oocyte development in Cdk2-/- mice
12. Expression of cell cycle regulators
13. Kinase activity and Cdk/cyclin complexes
14. Growth defect in Cdk2-/- MEFs
15. Cdk complexes and activity in MEFs
16. Cdk2-/- MEF analysis after starvation
17. Cdk activity in synchronized MEFs
18. Delayed immortalization in Cdk2-/- MEFs
19. Summary Cdk2
20. Is there another gene that compensates for Cdk2?
21. The Retinoblastoma (Rb) protein pathway
22. Cdk2-/-Cdk4-/- double knockout mice
23. Double mutants die around E15
24. Heart defects in Cdk2-/-Cdk4-/- embryos
25. In vivo proliferation in Cdk2-/-Cdk4-/- embryos
26. Hypophosphorylated Rb
27. Impaired proliferation in Cdk2-/-Cdk4-/- MEFs
28. S phase entry defect in Cdk2-/-Cdk4-/- MEFs
29. Rb phosphorylation in Cdk2-/-Cdk4-/- MEFs
30. Rb defect in Cdk2-/-Cdk4-/- MEFs
31. Senescence in Cdk2-/-Cdk4-/- MEFs
32. HPV-E7
33. HPV-E7 mutants
34. Effect of Cdk2-/-Cdk4-/- on Cdk1 expression
35. Summary Cdk2-/-Cdk4-/-
36. What is going on in the S phase?
37. The p27 network
38. Cdk2-/-p27-/- double knockout mice
39. Cdk2-/- and p27-/- mice vs. WT
40. Cdk2-/-p27-/- mice
41. Ovary tumors in Cdk2-/-p27-/- mice
42. Pituitary tumors
43. Normal levels of cell death in the thymus
44. High levels of S phase and mitosis in thymus
45. Cdk activity in the absence of Cdk2 and p27
46. Cdk1 binds to p27 and cyclin E
47. Proliferation of MEFs
48. Silencing of Cdk1 in Cdk2-/- MEFs
49. Summary Cdk2-/-p27-/-
50. Cdk1/cyclin E complexes can drive S phase
51. Cdk1 compensates for Cdk2
52. Premature translocation to the nucleus of Cdk1
53. DNA damage response in Cdk2-/- knockout mice
54. Response to gamma-irradiation in MEFs (1)
55. Response to gamma-irradiation in MEFs (2)
56. Partial hepatectomy model for in vivo response
57. Molecular outcome of gamma-irradiation
58. Co-localization of Cdk1 and p21
59. Proliferation of MEFs after irradiation
60. Cdk2-/- mice are sensitive to gamma-irradiation
61. DNA damage: comet assay
62. DNA damage: gamma-H2AX staining
63. Disturbed kinetics of DNA damage foci
64. DNA damage pathway
65. DNA damage in the absence of Cdk2
66. Acknowledgments

Topics Covered

• Cell cycle regulation during mouse development and adulthood
• Cyclin-dependent kinases (Cdks)
• The genetic pathways for cell cycle regulation
• Cdk1, Cdk2, Cdk4, and Cdk6
• The in vivo functions of Cdk2
• Compensation between different Cdk/cyclin complexes
• Genetic interaction between Cdk2 and Cdk4
• Control of the G1/S phase transition by the Retinoblastoma protein (Rb)
• DNA damage response in the absence of Cdk2
• p27Kip1 inhibits Cdk1 in vivo
• Cdk1/cyclin E complexes promote S phase
• Cdks and tumors

Links

Series:

• The Cell Division Cycle

Categories:

• Cancer
• Cell Biology

Therapeutic Areas:

• Oncology

Talk Citation

Kaldis, P. (2009, April 30). Mouse models to investigate cell cycle and cancer [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved January 29, 2020, from https://hstalks.com/bs/1269/.

Publication History

• Published on April 30, 2009

Financial Disclosures

• Dr. Philipp Kaldis has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.