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- Co-ordination of G1 Progression
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1. START control in yeast
- Prof. Curt Wittenberg
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2. The pRB/E2F pathway
- Prof. Jacqueline Lees
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3. Cell cycle control by the ubiquitin system in mammals
- Prof. Michele Pagano
- Chromosome Duplication
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4. Replication licensing
- Prof. Julian Blow
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5. Initiation of DNA replication
- Prof. Bruce Stillman
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6. Regulation of replication fork progression and stability
- Dr. Luis Aragón
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7. Nucleosome assembly during DNA replication
- Dr. Alain Verreault
- Preparing for Mitosis
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8. Sister chromatid cohesion: simple concept, complex reality
- Prof. Douglas Koshland
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9. Mitotic chromosome condensation
- Prof. Andrew Belmont
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10. Centrosome duplication and separation in animal cells
- Prof. Andrew Fry
- Spindle Assembly and Chromosome Segregation
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11. Bipolar spindle assembly
- Dr. Eric Karsenti
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12. Chromosome bi-orientation in yeast
- Prof. Mike Stark
- Prof. Tomo Tanaka
- Mitotic Exit and Cytokinesis
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14. Cleavage furrow formation and ingression during animal cytokinesis
- Dr. Pier Paolo D'Avino
- Checkpoints Governing Cell Cycle Progression
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15. The DNA damage response
- Dr. Vincenzo Costanzo
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16. The spindle checkpoint
- Dr. Kevin Hardwick
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17. Spindle movement and checkpoint control during mitosis in yeast
- Prof. John Cooper
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18. The G2/M transition
- Prof. Dr. René Medema
- The Cell Cycle in Development and Cancer
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19. Mouse models to investigate cell cycle and cancer
- Dr. Philipp Kaldis
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20. Cell cycle: a complex network of signals regulating cell proliferation
- Prof. Antonio Giordano
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21. Drug discovery and target validation in the p53 pathway
- Prof. Sir David Lane
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22. Role and regulation of Cdk inhibitors in development and cancer
- Prof. Martine Roussel
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24. The Myc transcription factor network
- Prof. Robert N. Eisenman
- Meiosis: A Specialized Cell Cycle
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25. Recombination and the formation of chiasmata in meiosis
- Prof. Matthew Whitby
- Archived Lectures *These may not cover the latest advances in the field
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26. Geometric regulation of kinetochore orientation
- Prof. Yoshinori Watanabe
Printable Handouts
Navigable Slide Index
- Introduction
- Cell cycle control of DNA replication
- Yeast as a model eukaryote
- Control of S phase entry in yeast
- Characterization of origins of DNA replication
- Modular structure of S. cerevisiae origins
- The budding yeast origin recognition complex
- Structure of the ORC-Cdc6 complex
- Evolutionary comparison of ORC AAA+ subunits
- DnaA and DnaC recognize OriC and load DnaB
- Formation of the pre-RC
- Association of initiation proteins with chromatin
- ATP dependent assembly of the pre-RC
- Cell cycle regulation of the pre-RC
- Chromosome cycle and establishment of pre-RCs
- Dynamics of ORC in human cells
- Commitment to S phase and initiation
- Cdc45p is associated with chromatin
- Cdc45p ChIP during S phase
- CDK and DDK-dependent assembly of pre-IC
- By-pass of CDK for pre-IC assembly
- Temporal regulation of DNA replication
- Dynamics of pre-IC in human cells
- Dynamics of ORC and Cdc6
- Orc2 and the cell cycle
- Initiation of DNA synthesis - the fork
- SV40: a simple replicon
- DNA replication-dependent chromatin assembly
- Initiation of virus DNA replication
- The "primasome" loading
- E1 helicase activity on single strand DNA (1)
- E1 helicase activity on single strand DNA (2)
- PCNA as a DNA replication protein
- Temporal organization of DNA replication
- PCNA, MCM3 during cell division cycle
- Conserved structure of polymerase clamps
- Sequential events at the SV40 DNA replication fork
- SV40 DNA replication fork
- Eukaryotic cell DNA replication fork
- Challenges for the future
Topics Covered
- Cell cycle control of DNA replication
- Yeast as a model eukaryote
- Control of S-phase entry in yeast
- Characterization of origins of DNA replication
- Modular structure of S. cerevisiae origins
- The budding yeast origin recognition complex (ORC)
- Structure of the ORC-Cdc6 complex
- Evolutionary comparison of ORC AAA+ subunits
- Proteins required for formation of the pre-Replication Complex (pre-RC)
- Cell cycle dependent association of yeast initiation proteins with chromatin
- ATP-dependent assembly of a pre-RC
- Cell cycle regulation of the pre-RC
- The chromosome cycle and establishment of pre-RCs
- Dynamic assembly and stability of ORC in human cells
- Commitment to S-phase and initiation
- Cdc45p is associated with chromatin in a cell cycle-dependent manner
- Temporal regulation of DNA replication
- Dynamics of pre-RC proteins in human cells differ from pre-RC proteins in yeast
- Dynamics of human cell ORC and Cdc6 during the cell division cycle
- Orc2 and the cell cycle
- Initiation of DNA synthesis: the fork
- SV40: a simple replicon
- DNA replication-dependent chromatin assembly
- Initiation of virus DNA replication
- Initiation of DNA synthesis
- E1 helicase activity on single stranded DNA
- Identification of PCNA as a DNA replication protein
- Temporal organization of DNA replication in human cell nuclei
- Conserved structure (not sequence) of polymerase clamps
- Sequential events at the SV40 DNA replication fork
- Proteins at the SV40 DNA replication fork
- Proteins at the eukaryotic cell DNA replication fork
- Challenges for the future
Talk Citation
Stillman, B. (2009, May 5). Initiation of DNA replication [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved March 29, 2024, from https://hstalks.com/bs/1257/.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Bruce Stillman has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.