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1. Introduction
2. Ensuring precise chromosome duplication
3. Activities required to control the duplication
4. Outline
5. The replication licence
6. Somatic cell fusion
7. Evidence for a replication licensing factor
8. Mcm2-7
9. The pre-replicative complex
10. Mcm4 in HeLa nuclei
11. Structure of Mcm2-7
12. Do Mcm2-7 provide the fork helicase?
13. The licensing reaction
14. Other pre-RC proteins
15. Origin recognition complex
16. Cdc6
17. Cdt1
18. Licensing chromatin with purified proteins
19. Licensing of replication origins
20. Cell cycle regulation of replication licensing
21. The licensing cycle
22. Result of mitotic CDKs inactivation in S. pombe
23. Pre-RC substrates of CDKs in yeast
24. The licensing cycle in S. cerevisiae
25. Cdt1 regulation in metazoa
26. Geminin - a metazoan licensing inhibitor
27. The licensing cycle in metazoan somatic cells
28. Licensing control in Xenopus eggs/embryos (1)
29. The licensing cycle in Xenopus embryos
30. Licensing control in Xenopus eggs/embryos (2)
31. Role of the ELYS-Mcm2-7 link
32. The licensing system and cancer
33. The 'MCM paradox'
34. Dormant origins
35. The license is lost on exit from the cell cycle
36. Mcm2-7 are down-regulated on cell cycle exit
37. Cervical carcinoma detection by Mcm5 staining
38. Licensing defects induced by oncogenes
39. Checkpoint in primary cell lines / cancer cell lines

Topics Covered

• Problems in ensuring complete genome replication
• Experiments revealing the existence of a replication licence
• The licensing reaction
• ORC, Cdc6, Cdt1 and Mcm2-7
• Cell cycle regulation of licensing
• Pre-RC substrates in yeast
• Regulation of licensing in animal cells by Cdt1 degradation and geminin inhibition
• Is there a licensing checkpoint?
• Replication licensing and cancer
• The 'MCM paradox' and dormant replication origins
• The licensing system as a potential anti-cancer target

Links

Series:

• The Cell Division Cycle

Categories:

• Cell Biology
• Genetics & Epigenetics

Talk Citation

Publication History

• Published on April 30, 2009

Financial Disclosures

• Prof. Julian Blow has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.