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- Co-ordination of G1 Progression
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1. START control in yeast
- Prof. Curt Wittenberg
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2. The pRB/E2F pathway
- Prof. Jacqueline Lees
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3. Cell cycle control by the ubiquitin system in mammals
- Prof. Michele Pagano
- Chromosome Duplication
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4. Replication licensing
- Prof. Julian Blow
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5. Initiation of DNA replication
- Prof. Bruce Stillman
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6. Regulation of replication fork progression and stability
- Dr. Luis Aragón
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7. Nucleosome assembly during DNA replication
- Dr. Alain Verreault
- Preparing for Mitosis
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8. Sister chromatid cohesion: simple concept, complex reality
- Prof. Douglas Koshland
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9. Mitotic chromosome condensation
- Prof. Andrew Belmont
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10. Centrosome duplication and separation in animal cells
- Prof. Andrew Fry
- Spindle Assembly and Chromosome Segregation
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11. Bipolar spindle assembly
- Dr. Eric Karsenti
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12. Chromosome bi-orientation in yeast
- Prof. Mike Stark
- Prof. Tomo Tanaka
- Mitotic Exit and Cytokinesis
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14. Cleavage furrow formation and ingression during animal cytokinesis
- Dr. Pier Paolo D'Avino
- Checkpoints Governing Cell Cycle Progression
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15. The DNA damage response
- Dr. Vincenzo Costanzo
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16. The spindle checkpoint
- Dr. Kevin Hardwick
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17. Spindle movement and checkpoint control during mitosis in yeast
- Prof. John Cooper
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18. The G2/M transition
- Prof. Dr. René Medema
- The Cell Cycle in Development and Cancer
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19. Mouse models to investigate cell cycle and cancer
- Dr. Philipp Kaldis
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20. Cell cycle: a complex network of signals regulating cell proliferation
- Prof. Antonio Giordano
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21. Drug discovery and target validation in the p53 pathway
- Prof. Sir David Lane
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22. Role and regulation of Cdk inhibitors in development and cancer
- Prof. Martine Roussel
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24. The Myc transcription factor network
- Prof. Robert N. Eisenman
- Meiosis: A Specialized Cell Cycle
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25. Recombination and the formation of chiasmata in meiosis
- Prof. Matthew Whitby
- Archived Lectures *These may not cover the latest advances in the field
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26. Geometric regulation of kinetochore orientation
- Prof. Yoshinori Watanabe
Printable Handouts
Navigable Slide Index
- Introduction
- Ubiquitin-proteasome and cell cycle progression
- What regulates the cell division cycle?
- Cyclin-dependent kinases
- Cdk1, Cdk2 and their regulators
- Regulated proteolysis is unidirectional
- Regulation by phosphorylation vs. proteolysis
- Advantages of regulated proteolysis
- The Ubiquitin-proteasome system
- The hierarchy of the ubiquitin pathway
- E2s and E3s ensure substrate specificity
- Regulation of the cell cycle by proteolysis
- Two major ubiquitin ligases control cell cycle
- SCF ubiquitin ligases
- Role of F-box proteins in the SCF complex
- Spatial arrangement of the SCF complex
- Mechanism of catalysis by the SCF
- Super-enzyme vs. typical enzyme
- The various F-box proteins in human
- 5 F-box proteins and their respective substrates
- Skp2 (Fbxl1)
- Skp2 is a positive regulator of CDKs
- Targeting of p21 to degradation by APC/C-Cdc20
- Beta-Trcp1 (Fbxw1) and beta-Trcp2 (Fbxw11)
- Function of beta-Trcp during replication stress
- beta-Trcp, after recovery from the replication stress
- Negative and positive regulation by beta-Trcp
- Fbxw7 (Fbw7 or Cdc4)
- Fbxw7 is a negative regulator of CDKs (when?)
- APC/C (anaphase promoting complex/cyclosome)
- APC/C contribution to cell cycle progression
- Activity of APC/C containing Cdc20 or Cdh1
- SCF controls mitosis by regulating APC/C activity
- APC/C controls G1 by regulating Skp2 stability
- APC/C-Cdh1 is mainly active in G1
- APC/C-Cdh1 activated in G2 after DNA damage
- Ubiquitin system and cell cycle - summary (1)
- Ubiquitin system and cell cycle - summary (2)
- Complexity of the system
- Cell cycle, ubiquitin system and cancer
- Proto-oncoprotein and tumor-suprresors
- Skp2 is a positive regulator of CDKs
- Skp2 is a proto-oncoprotein
- Tumor suppressors targeted by Skp2
- Fbxw7 is a negative regulator of CDKs
- Fbxw7 is a tumor suppressor
- Proto-oncoproteins targeted by Fbxw7
- beta-Trcp is a negative & positive CDKs regulator
- beta-Trcp is a proto-oncoprotein
- Cdh1 is a tumor suppressor
- Concluding remarks
Topics Covered
- Ubiquitin-proteasome system
- What regulates the cell division cycle?
- Cyclin dependent kinases
- Advantages of regulated proteolysis
- The hierarchy of the ubiquitin pathway
- E2s and E3s ensure substrate specificity
- Regulation of the cell cycle by proteolysis
- Two major ubiquitin ligases control cell cycle progression
- SCF ubiquitin ligases
- Mechanism of catalysis by the SCF
- F-box proteins
- Skp2
- betaTrcp1 and betaTrcp2
- DNA replication stress
- Fbxw7
- Anaphase promoting complex or cyclosome
- Cell cycle, ubiquitin system and cancer
Talk Citation
Pagano, M. (2009, April 30). Cell cycle control by the ubiquitin system in mammals [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 20, 2024, from https://hstalks.com/bs/1255/.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Michele Pagano has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
Cell cycle control by the ubiquitin system in mammals
Published on April 30, 2009
35 min