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1. Introduction
2. Ubiquitin-proteasome and cell cycle progression
3. What regulates the cell division cycle?
4. Cyclin-dependent kinases
5. Cdk1, Cdk2 and their regulators
6. Regulated proteolysis is unidirectional
7. Regulation by phosphorylation vs. proteolysis
8. Advantages of regulated proteolysis
9. The Ubiquitin-proteasome system
10. The hierarchy of the ubiquitin pathway
11. E2s and E3s ensure substrate specificity
12. Regulation of the cell cycle by proteolysis
13. Two major ubiquitin ligases control cell cycle
14. SCF ubiquitin ligases
15. Role of F-box proteins in the SCF complex
16. Spatial arrangement of the SCF complex
17. Mechanism of catalysis by the SCF
18. Super-enzyme vs. typical enzyme
19. The various F-box proteins in human
20. 5 F-box proteins and their respective substrates
21. Skp2 (Fbxl1)
22. Skp2 is a positive regulator of CDKs
23. Targeting of p21 to degradation by APC/C-Cdc20
24. Beta-Trcp1 (Fbxw1) and beta-Trcp2 (Fbxw11)
25. Function of beta-Trcp during replication stress
26. beta-Trcp, after recovery from the replication stress
27. Negative and positive regulation by beta-Trcp
28. Fbxw7 (Fbw7 or Cdc4)
29. Fbxw7 is a negative regulator of CDKs (when?)
30. APC/C (anaphase promoting complex/cyclosome)
31. APC/C contribution to cell cycle progression
32. Activity of APC/C containing Cdc20 or Cdh1
33. SCF controls mitosis by regulating APC/C activity
34. APC/C controls G1 by regulating Skp2 stability
35. APC/C-Cdh1 is mainly active in G1
36. APC/C-Cdh1 activated in G2 after DNA damage
37. Ubiquitin system and cell cycle - summary (1)
38. Ubiquitin system and cell cycle - summary (2)
39. Complexity of the system
40. Cell cycle, ubiquitin system and cancer
41. Proto-oncoprotein and tumor-suprresors
42. Skp2 is a positive regulator of CDKs
43. Skp2 is a proto-oncoprotein
44. Tumor suppressors targeted by Skp2
45. Fbxw7 is a negative regulator of CDKs
46. Fbxw7 is a tumor suppressor
47. Proto-oncoproteins targeted by Fbxw7
48. beta-Trcp is a negative & positive CDKs regulator
49. beta-Trcp is a proto-oncoprotein
50. Cdh1 is a tumor suppressor
51. Concluding remarks

Topics Covered

• Ubiquitin-proteasome system
• What regulates the cell division cycle?
• Cyclin dependent kinases
• Advantages of regulated proteolysis
• The hierarchy of the ubiquitin pathway
• E2s and E3s ensure substrate specificity
• Regulation of the cell cycle by proteolysis
• Two major ubiquitin ligases control cell cycle progression
• SCF ubiquitin ligases
• Mechanism of catalysis by the SCF
• F-box proteins
• Skp2
• betaTrcp1 and betaTrcp2
• DNA replication stress
• Fbxw7
• Anaphase promoting complex or cyclosome
• Cell cycle, ubiquitin system and cancer

Links

Series:

• The Cell Division Cycle

Categories:

• Biochemistry
• Cell Biology

Talk Citation

Pagano, M. (2009, April 30). Cell cycle control by the ubiquitin system in mammals [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 19, 2024, from https://hstalks.com/bs/1255/.
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Publication History

• Published on April 30, 2009

Financial Disclosures

• Prof. Michele Pagano has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.