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1. Germ cells
2. Fertilized egg
3. Mammalian egg
4. Fertilization
5. Parental mammalian genomes - non-equivalent
6. Properties of germ cell lineage
7. Mechanisms of germ cell specification
8. Germ line cycle in mouse
9. Mechanism of PGC specification in mice
10. Specification and demarcation of PGCs
11. Genetic basis of germ cell specification in mice
12. Single cell cDNAs and somatic neighbours
13. Genes involved in PGC specification
14. fragilis/stella expression during PGC specification
15. Mechanism of PGC specification in mice
16. Mechanism of somatic programme repression
17. HMTases in single PGCs and somatic neighbours
18. Blimp1: transcrip. regulator/cell fate determinator
19. Blimp1: regulator of PGC specification in mice
20. Blimp1: expression starts earlier than stella
21. Blimp1: expressed early during development
22. Blimp1: no. of positive cells during development
23. Blimp1: positive cells are lineage restricted PGCs
24. Evidence for role of Blimp1 in PGC specification
25. Blimp1: analysis in PGC specification
26. Loss of Blimp1 causes loss of founder PGCs
27. Aberrant phenotype of Blimp1 deficient cells
28. Analysis of single PGCs and somatic neighbours
29. Summary: key points in PGC specification in mice
30. Potential mechanisms of repression by Blimp-1
31. Epigenetic programming in gonadal PGCs
32. Epigenetic modifications after PGC specification
33. Nanog expression in Stella positive nascent PGCs
34. stella/nanog/Gdf3 are linked in mouse and human
35. Key histone modifications after PGC specification
36. Germ line reprogramming
37. Genetic programme for specification of PGC fate
38. PGCs and pluripotent SCs: reversible phenotypes
39. Epigenetic programming in embryos and PGCs
40. Conclusions
41. Acknowledgements

Topics Covered

• The germ cell cycle and the key epigenetic programming events in the germ cell lineage
• the oocyte and its properties
• epigenetic mechanism of germ cell specification in mice
• repression of the somatic programme, a key event during specification of germ cells
• epigenetic programming following specification of germ cells
• the relationship between germ cells and pluripotent stem cells

Links

Series:

• Epigenetics

Categories:

• Biochemistry
• Cell Biology
• Genetics & Epigenetics
• Reproduction & Development

Therapeutic Areas:

• Gynaecology & Obstetrics

Talk Citation

Publication History

• Published on October 1, 2007
• Archived on May 31, 2018

Financial Disclosures

• Prof. Azim Surani has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.