• The Basics of Microarrays
• 42 min
  1. How to design a successful microarray experiment

  • Dr. Rainer Breitling
• Microarrays in the Understanding of the Biology of the Cell and Whole Organism
• 51 min
  2. Genome-wide mapping of estrogen: receptor binding sites and activity

  • Prof. Jason Carroll
• Microarrays in the Diagnosis, Prognosis and Treatment of Disease
• 35 min
  3. Single nucleotide polymorphism microarrays in the analysis of cancer

  • Dr. Rameen Beroukhim
• Archived Lectures *These may not cover the latest advances in the field
• 23 min
  4. The use of statistics in microarray studies

  • Dr. Ernst Wit
• 42 min
  5. Simultaneous microarray measurement of nuclear and whole cell changes in gene expression reveals overlapping but distinct patterns of transcriptional regulation

  • Dr. Chris Cheadle
• 38 min
  6. Global analysis of gene translation

  • Prof. Philip Tofilon
• 36 min
  7. Microarrays in the study of the basic mechanisms of evolution

  • Dr. Greg Gibson
• 41 min
  8. The use of microarrays to understand the biology and toxicology of low dose arsenic

  • Prof. Joshua Hamilton
• 46 min
  9. The bioinformatics of microarrays

  • Dr. Maureen Sartor
• 44 min
  10. Genome-wide profiling of microRNAs: from scientist benchside to patient bedside

  • Prof. George Calin
• 42 min
  11. Microarrays: their design and use

  • Prof. Craig R. Tomlinson
• 67 min
  12. Cancer systems biology: dissection and analysis of context-specific molecular interaction networks

  • Prof. Andrea Califano
• 35 min
  13. Utilizing microarrays to study posttranscriptional regulatory mechanisms of plants

  • Dr. Brian Gregory

Printable Handouts

PDF

Navigable Slide Index

1. Introduction
2. Outline
3. Scope and introduction
4. Genomics
5. Genomics domain
6. Target audience (1)
7. Genomic information in complex biologic systems
8. Target audience (2)
9. What are microarrays?
10. Themes incorporated in microarray technology
11. What is a microarray?
12. What is the basic way microarrays are carried out
13. Competitive or dual hybridization
14. Phases in a microarray experiment - phase 1
15. Experimental design
16. Phases in microarray experiment - phase 2
17. Technical and biological replication
18. RNA quality control
19. Phases in microarray experiment - phase 3
20. Data analysis
21. The microarray platforms available
22. Different microarray platforms
23. Microarrays to examine differential RNA levels
24. Agilent technologies
25. Agilent target labeling
26. An Agilent array
27. Results from an Agilent array
28. Affymetrix
29. Affymetrix: single target hybridization
30. The photolithography method
31. Affymetrix GeneChip array
32. Target production and labeling
33. Illumina
34. Illumina's BeadArray technology
35. Direct hybridization RNA profiling bead
36. Comparative genomic hybridization (CGH)
37. CGH description
38. CGH data (1)
39. CGH data (2)
40. CpG island methylation
41. Hypomethylated fraction
42. ChIP-on-chip
43. ChIP-on-chip methods
44. miRNA (1)
45. miRNA (2)
46. miRNA (3)
47. Tiling arrays
48. Tiling arrays - description
49. Speaker introductions
50. The basics of microarrays
51. The basics of microarrays - Rainer Breitling
52. The basics of microarrays - Ernst Wit
53. The basics of microarrays - Maureen Sartor
54. Microarrays in cell and whole organism biology
55. Cell and organism biology - Chris Cheadle
56. Cell and organism biology - Jason Carroll
57. Cell and organism biology - Philip Tofilon
58. Microarrays and disease
59. Microarrays and disease - Rameen Beroukhim
60. Microarrays and disease - George Calin
61. Evolution, ecology, and toxicology
62. Evolution, ecology, and toxicology - Greg Gibson
63. Evolution, ecology, and toxicology - Xiaoyu Zhang
64. Joshua W. Hamilton
65. The future of microarray technology
66. The future - Andrea Califano
67. Concluding remarks
68. The End

Topics Covered

• The use of microarrays to study genomic DNA and expressed RNA at a global level are being developed at a phenomenal pace in the basic and medical sciences
• The basics of microarrays
• Fundamental descriptions and issues regarding experimental design, high-throughput approaches, analysis, and bioinformatics
• Different microarray platforms can be used to examine single nucleotide polymorphisms (SNP) of the genome, differential mRNA levels, differential microRNA (miRNA) levels, DNA methylation, genomic deletions and additions, DNA binding sites, and transcription rates
• Microarrays in the diagnosis, prognosis, and treatment of disease
• Microarrays in evolution, ecology and toxicology
• The future of microarray technology

Links

Series:

• Microarrays

Categories:

• Genetics & Epigenetics
• Methods

Talk Citation

Publication History

• Published on March 30, 2009
• Archived on February 25, 2021

Financial Disclosures

• Prof. Craig R. Tomlinson has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.