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Navigable Slide Index

1. Intro slide
2. Chromatin packaging: the static view
3. Chromatin: the dynamic view
4. Building and regulating chromatin
5. Loci that utilize chromatin dynamics
6. Chromatin remodelers in dynamic chromatin
7. Dynamic nucleosome repositioning
8. ISWI and RNA Pol II are mutually exclusive
9. BRM and RNA Pol II are largely coincident
10. Loss of BRM function halts transcription
11. Localization of BRM/PC complexes are exclusive
12. Dynamic nucleosome repositioning
13. The yeast remodelers: SWI/SNF and RSC
14. Remodelers slide nucleosomes in-vitro
15. Dynamic nucleosomes in-vivo
16. Composition of SWI/SNF and ISWI
17. Mechanism of chromatin remodeling: questions
18. RSC and its catalytic subunit Sth1
19. The nucleosome
20. The Half Nucleosome
21. DNA translocation: preview
22. The DNA translocation/wave model
23. Mechanism of chromatin remodeling: questions
24. 2-dimensional projection of the nucleosome
25. Exo III mapping with RSC
26. DNase I mapping of the nucleosome
27. RSC envelops the nucleosome
28. RSC/Sth1 induce a hypersensitive site
29. Sth1 binds internally
30. 2 Sth1 binding orientations
31. Sth1 binds at 1 of 2 sites
32. Sth1 binds nucleosome about 2 turns from dyad
33. ISWI binds nucleosomal DNA at 2 locations
34. RSC/Sth1 binding: summary
35. Mechanism of chromatin remodeling: questions
36. Sth1 pumps DNA around the nucleosome
37. DNA translocases
38. Evidence for translocation
39. Triplex displacement model
40. Triplex displacement by RSC
41. Directional DNA translocation on the nucleosome
42. Predicted products of Sth1/RSC translocation
43. RE cleavage assay for DNA translocation
44. RE exposure prediction
45. RE cleavage with linkers
46. Reciprocal linker prediction
47. Gapped nucleosome prediction
48. Gapped nucleosome data: Dra I
49. Gapped nucleosome data: Fok I
50. Gapped nucleosome data: Msp I
51. A model for DNA translocation on nucleosomes
52. Remodelers reposition and transfer nucleosomes
53. RSC catalyzes nucleosome transfer
54. Nucleosome transfer by DNA translocation
55. Remodeler observations
56. Model for factor access on nucleosome
57. Chromatin remodelers in dynamic chromatin
58. SWR1 complex
59. Chromatin remodelers in dynamic chromatin
60. Acknowledgements

Topics Covered

• ATP-dependent chromatin remodeling complexes
• ATP hydrolysis and nucleosome dynamics
• Mechanistic action
• Cellular function

Links

Series:

• Eukaryotic Gene Regulation

Categories:

• Biochemistry
• Cell Biology
• Genetics & Epigenetics

Talk Citation

Cairns, B. (2007, October 1). Dynamic chromatin: ATP-dependent chromatin remodeling machines [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved December 27, 2024, from https://doi.org/10.69645/WSKS5835.
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Publication History

• Published on October 1, 2007
• Archived on April 13, 2022

Financial Disclosures

• Prof. Bradley Cairns has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.