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0:00
Welcome everybody to this
talk on the regulation of
enzyme activity by
inhibitors and activators.
My name is Antonio Baici,
retired from the
University of Zurich.
I have been active in the
field of enzyme kinetics,
investigating the
theoretical aspects
of enzyme inhibition
with obligations to
pathological degradation
of connective tissues.
0:25
In this talk, I will
bring to your attention
the kinetic groundwork of
enzyme modifier interruptions.
I will start with
the general modifier mechanism
and some principles
of enzyme kinetics.
The present nomenclature of
enzyme inhibitors and
activators is answered
dissatisfactorally, this is partly
due to the non observance of
the recommendations issued by
the International Union of
biochemistry and
Molecular Biology and
partly to merging
unrelated mechanisms
under collected names.
I will describe a
systematics treatment
of modifier mechanisms,
which is entirely based on
the recommendations and on
the general modifier mechanism.
Don't worry for names, concepts,
and the definitions
possibly new to you.
You can re-examine them on links
I will show in the last slide.
The intended purpose
of this talk
is to illustrate a
logical approach to
the investigation of enzyme
modified interactions with
emphasis on allosteric
regulation.
1:33
Jean Botts and her husband
Manuel Francisco Morales,
wrote a fundamental paper on
enzyme modifier
interactions. The term
modify in the title
declares the intention of
treating every kind of
enzyme inhibition and
activation under the same roof.
An important statement
at the beginning of
the mathematical
derivation seems to have
been underestimated by
the scientific community.
For the discussion
of this effect,
one may visualize and
enzymatic patch upon
the enzyme molecule,
consisting of an enzymatic site
which bind substrate molecules,
and then adjacent side which
binds modifier molecules.
The highlighted adjacent
side is evidently
a less spectacular definition
of allostatic site,
published 10 years later in
the famous paper by
mono and co-workers.
Moreover, Botts and Morales
published a complete
mathematical treatment,
not found on all papers.
