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Printable Handouts
Navigable Slide Index
- Introduction
- Endoplasmic reticulum
- Mitochondria
- How to measure organelle Ca?
- Cameleon probes for Ca measurements (1)
- Cameleon probes for Ca measurements (2)
- ApoK1er: a probe for Ca measurements
- Ca dynamics in the cytosol, ER and mitochondria
- Ca crosstalk between the ER and the mitochondria
- Importance of mitochondrial Ca
- Ca regulation of mitochondrial ATP biosynthesis
- Principle parameters for the Ca flux
- Biophysical conditions: membrane potential
- FCCP depolarizing the membrane
- Effect of depolarizing agent on Ca flux
- Morphological aspects: interorganelle distance
- Impact of interorganelle distance of Ca crosstalk
- Morphological aspects: interacting surface area
- Colocalization of ER and mitochondria
- Morphological aspects: organelle integrity
- Organelle integrity - analyses
- Biological keystone: IP3 receptor, VDAC
- Biological keystone: mitochondrial uniporter
- Mitochondrial uniporter - references
- Functional parameters: mitochondrial uptake
- Phases of mitochondrial uptake
- The extrusion phase - NCX
- The extrusion phase - SERCA pump
- Mitochondrial Ca extrusion is coupled with SERCA
- Mitochondria fragmentation effect on Ca crosstalk
- Mitochondria fragmentation delays ER Ca refilling
- The transit phase (1)
- The Ca paradox
- Inner high Ca inhibits Ca/K pathway
- Ca/K channel dramatically affects Ca entry
- Subplasmalemmal Ca gradients establishment
- Measuring local Ca gradients beneath the PM
- Measuring with patch clamp
- ER and mitochondria create opposite Ca gradients
- The transit phase (2)
- Interorganelle Ca crosstalk
- Thank you
Topics Covered
- 3D structure of the endoplasmic reticulum and mitochondria
- Techniques to measure organelle calcium
- Mitochondrial functions
- Principles of mitochondrial calcium signaling
- Functional coupling of the endoplasmic reticulum and the mitochondria
- Consequences of alterations in the structural integrity of the mitochondria
- Subplasmalemmal mitochondrial calcium buffering
- Physiological importance of mitochondrial calcium uptake
- The cellular calcium cycle
Talk Citation
Graier, W. (2007, October 1). Ca2+ dynamics between mitochondria and the endoplasmic reticulum [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved August 25, 2024, from https://doi.org/10.69645/AXJM4702.Export Citation (RIS)
Publication History
Financial Disclosures
- Dr. Wolfgang Graier has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.