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1. Introduction
2. Lecture structure
3. Renewed interest in viral respiratory tract infections
4. Human respiratory viral infections
5. Viral pneumonia in adults (New Zealand)
6. Diagnostic tools for respiratory viruses
7. Electron microscopy
8. Direct immunofluorescence
9. Cell culture
10. Antibody detection (serology)
11. Rapid antigen tests
12. Accuracy of rapid influenza diagnostic tests (1)
13. Accuracy of rapid influenza diagnostic tests (2)
14. Accuracy of rapid influenza diagnostic tests (3)
15. Main conclusion from the study
16. RSV rapid tests compared with cell culture
17. Nucleic acid detection tests
18. PCR time line
19. Nucleic acid detection in respiratory infections
20. PCR: the test of choice for respiratory viruses
21. Respiratory viruses in nasopharyngeal samples
22. Characteristics of different diagnostic tests
23. Time line of respiratory viral diagnostics
24. Ongoing improvements in viral diagnostics
25. Better antigen detection tests
26. Developments in nucleic acid detection methods
27. List of assays for respiratory viruses
28. Conventional real-time PCR
29. xTAG® respiratory viral panel FAST
30. TaqMan array card (TAC) (1)
31. TaqMan array card (TAC) (2)
32. FilmArray technology
33. GeneXpert test for influenza
34. Evaluation of multiplex assays
35. PCR quantification
36. Viral load is related to clinical diagnosis (1)
37. Viral load is related to clinical diagnosis (2)
38. Mass spectrometry in clinical microbiology
39. MassCode PCR
40. PLEX-ID technology (1)
41. PLEX-ID technology (2)
42. More sophisticated nucleic acid testing methods
43. DNA microarrays (1)
44. Typical microarray process
45. Utility of DNA microarrays for detection of viruses
46. DNA microarrays (2)
47. Recently discovered respiratory viruses
48. Growth of the viral sequence database
49. Staged strategy for pathogen discovery
50. High-throughput sequencing technology
51. Gene analysis in nasal samples
52. Host gene signatures
53. Gene expression signature
54. Neglected issues
55. Specimen collection
56. Viruses detected in children with pneumonia
57. Flocked swabs
58. Comparison of flocked swabs to other tests
59. Breath analysis
60. Low yield with breath analysis
61. Polymicrobial infections
62. Polymicrobial infections in pneumonia
63. Assigning causation
64. Some arithmetic …
65. Detection of respiratory pathogens by PCR
66. Summary
67. Acknowledgements

Topics Covered

• Human respiratory viral infections
• Community-acquired pneumonia in children
• Viral pneumonia in adults (New Zealand)
• Diagnostic tools for respiratory viruses their accuracy –and developments – Various assays for respiratory viruses
• Recently discovered respiratory viruses
• Growth of the viral sequence database – Details of staged strategy for pathogen discovery including tests, analysis of findings & assigning causation

Links

Series:

• Respiratory Infection

Categories:

• Clinical Practice
• Methods
• Microbiology

Therapeutic Areas:

• Infectious Diseases
• Respiratory Diseases

Talk Citation

Publication History

• Published on July 11, 2013

Financial Disclosures

• Prof. David Murdoch has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.