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Navigable Slide Index

1. Introduction
2. Stem cell bioprocessing: mission
3. Topics
4. Bioprocess environment
5. Bioprocess issues
6. Bioprocess operating space
7. Bioprocess culture parameters
8. Complex kinetics, transient nature
9. Complex interactions, growth medium
10. pH, dissolved oxygen tension
11. Haematopoietic cultures
12. Basic types of bioreactors
13. Stirred tank
14. Tubular reactors (plug flow)
15. Segregated and heterogeneous bioreactors
16. Operating mode of bioreactors
17. Surface cultures
18. Stirred tank bioreactors
19. Microcarrier systems
20. Packed bed bioreactors
21. Airlift bioreactors
22. Hollow-fibre bioreactors
23. Rotating wall vessel bioreactor
24. Bioreactor system design requirements
25. Design of experiments (DOE)
26. DOE goals and motivations
27. One-factor analysis
28. Results from DOE
29. Types of designs and experiments
30. Example: production of erythrocytes
31. Screening experiments
32. Process characterization
33. DOE cell culture issues and concerns
34. Summary of DOE

Topics Covered

• The development of bioprocessing technology: control, reproducibility, automation, validation and safety
• The bioprocessing environment
• Bioprocessing issues
• Bioprocessing operating system
• Bioprocessing culture parameters: media, pH, DO and cytokines
• Basic types of bioreactors: operating modes of bioreactors, bioreactor design criteria and bioreactor types
• Cell culture systems
• Bioreactor system design requirements
• Design of experiments (DOE)
• Types of designs and experiments
• DOE cell culture issues and concerns

Links

Series:

• Stem Cells and Regenerative Medicine

Categories:

• Cell Biology
• Methods

Talk Citation

Publication History

• Published on October 1, 2007

Financial Disclosures

• Dr. Sakis Mantalaris has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.