• Introduction
• 30 min
  1. Prions and amyloids: introduction

  • Prof. Reed Wickner
• Mammalian Prions
• 26 min
  2. Transgenic mouse models of prion diseases

  • Prof. Glenn Telling
• 31 min
  3. Mechanism of prion generation in vitro

  • Dr. Surachai Supattapone
• Non-Prion Amyloids
• 48 min
  4. Similarities between prion diseases and other neurodegenerative diseases

  • Prof. John Hardy
• 43 min
  5. Molecular structure of amyloid and prion fibrils: insights from solid state NMR

  • Dr. Robert Tycko
• Yeast Prions
• 42 min
  6. Chaperones and prions

  • Prof. Yury Chernoff
• Beneficial Amyloids
• 52 min
  7. The dark side of amyloid: PMEL, a natural amyloid in melanosome biogenesis

  • Prof. Michael Marks
• Archived Lectures *These may not cover the latest advances in the field
• 41 min
  8. Predicting TSE transmission

  • Prof. Jean Manson
• 53 min
  9. Generation and propagation of infectious prions by cyclic amplification of protein misfolding

  • Prof. Claudio Soto
• 30 min
  10. Yeast and fungal prions: a help or a hindrance?

  • Prof. Reed Wickner
• 42 min
  11. [PIN+]: prions beget prions

  • Prof. Susan Liebman
• 36 min
  12. Yeast prions and protein chaperones

  • Dr. Daniel Masison
• 54 min
  13. Mechanisms of yeast prion propagation

  • Prof. Mick Tuite
• 31 min
  14. Propagation and variability of the yeast [PSI+] prion

  • Prof. Michael Ter-Avanesyan
• 43 min
  15. The genetics and biology of the [Het-s] prion of Podospora

  • Prof. Sven Saupe
• 33 min
  16. The structure of amyloid fibrils of the HET-s (218-289) prion as seen by solid-state NMR

  • Prof. Beat Meier

Printable Handouts

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Navigable Slide Index

1. Introduction
2. Prion definition
3. Outline
4. Filamentous fungi form coenocytic structures
5. Filamentous fungi undergo somatic cell fusion
6. Heterokaryon incompatibility
7. Genetics of incompatibility
8. Proposed function of heterokaryon incompatibility
9. Positive Darwinian selection on het loci
10. The barrage reaction
11. The Het-s system (1)
12. The Het-s system (2)
13. Propagation of Het-s
14. [Het-s] shows strict maternal inheritance
15. [Het-s] is lost in het-s x het-S crosses
16. The het-S spore killing phenomenon
17. HET-s and HET-S differ by 13 amino acids
18. HET-s aggregates in vivo
19. HET-s forms self-perpetuating aggregates in vitro
20. HET-s aggregates are amyloids (1)
21. HET-s aggregates are amyloids (2)
22. HET-s amyloids display prion infectivity (1)
23. HET-s amyloids display prion infectivity (2)
24. HET-s amyloids display prion infectivity (3)
25. Proteinase K resistant HET-s amyloids core
26. Identification of pK resistant fragment
27. HET-s prion forming domain
28. Het-s prion forming domain in GFP fusion
29. HET-s structural transition analyses by HXMS (1)
30. Het-s structural transition analyses by HXMS (2)
31. Het-s structural transition analyses by HXMS (3)
32. Domain organisation of HET-s
33. Elongated Het-s 157-289 aggregates
34. Time lapse of Het-s 157-289 aggregate formation
35. In vivo EM of HET-s 157-289 aggregates
36. Het-s (218-289) structure (1)
37. Het-s (218-289) structure (2)
38. Het-s (218-289) structure (3)
39. Het-s (218-289) model
40. Het-s (218-289) structure-function correlation (1)
41. Het-s (218-289) structure-function correlation (2)
42. STEM and electron diffraction of HET-s fibrils
43. Prion and non-prion Het-s (218) amyloids (1)
44. Prion and non-prion Het-s (218) amyloids (2)
45. Prion and non-prion Het-s (218) amyloids (3)
46. Prion and non-prion Het-s (218) amyloids (4)
47. Prion and non-prion Het-s (218) amyloids (5)
48. Hsp104 chaperone role in Het-s prion propagation
49. PaHsp104 required for Podospora thermotolerance
50. Het-s propagated in the absence of PaHsp105
51. PaHsp104 inactivation reduces # propagons
52. PaHsp104 inactivation reduces propagation rate
53. PaHsp104 inactivation and aggregates morphology
54. PaHsp104 inactivation effect during sexual cycle
55. Het-s propagation in yeast (1)
56. Het-s propagation in yeast (2)
57. Summary
58. Comparison between Het-s and yeast prions
59. Open question (1)
60. Open question (2)
61. Acknowledgements
62. References

Topics Covered

• Mammalian prions
• Fungal prions
• Yeast: [URE3], [PSI+] and [PIN+]
• Filamentous fungi: [Het-s]
• Fungal self non-self recognition
• Heterokaryon incompatibility
• Genetics of [Het-s]
• Maternal inheritance
• Meiotic drive
• In vivo and in vitro aggregation of HET-s protein
• Role of the Hsp104 chaperone in [Het-s] system
• Role of the Hsp104 chaperone in [Het-s] prion propagation
• Podospora anserine

Links

Series:

• Prions and Amyloids

Categories:

• Biochemistry
• Cell Biology
• Genetics & Epigenetics
• Microbiology

Talk Citation

Publication History

• Published on September 4, 2008
• Archived on February 25, 2021

Financial Disclosures

• Prof. Sven Saupe has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.