• Introduction
• 30 min
  1. Prions and amyloids: introduction

  • Prof. Reed Wickner
• Mammalian Prions
• 26 min
  2. Transgenic mouse models of prion diseases

  • Prof. Glenn Telling
• 31 min
  3. Mechanism of prion generation in vitro

  • Dr. Surachai Supattapone
• Non-Prion Amyloids
• 48 min
  4. Similarities between prion diseases and other neurodegenerative diseases

  • Prof. John Hardy
• 43 min
  5. Molecular structure of amyloid and prion fibrils: insights from solid state NMR

  • Dr. Robert Tycko
• Yeast Prions
• 42 min
  6. Chaperones and prions

  • Prof. Yury Chernoff
• Beneficial Amyloids
• 52 min
  7. The dark side of amyloid: PMEL, a natural amyloid in melanosome biogenesis

  • Prof. Michael Marks
• Archived Lectures *These may not cover the latest advances in the field
• 41 min
  8. Predicting TSE transmission

  • Prof. Jean Manson
• 53 min
  9. Generation and propagation of infectious prions by cyclic amplification of protein misfolding

  • Prof. Claudio Soto
• 30 min
  10. Yeast and fungal prions: a help or a hindrance?

  • Prof. Reed Wickner
• 42 min
  11. [PIN+]: prions beget prions

  • Prof. Susan Liebman
• 36 min
  12. Yeast prions and protein chaperones

  • Dr. Daniel Masison
• 54 min
  13. Mechanisms of yeast prion propagation

  • Prof. Mick Tuite
• 31 min
  14. Propagation and variability of the yeast [PSI+] prion

  • Prof. Michael Ter-Avanesyan
• 43 min
  15. The genetics and biology of the [Het-s] prion of Podospora

  • Prof. Sven Saupe
• 33 min
  16. The structure of amyloid fibrils of the HET-s (218-289) prion as seen by solid-state NMR

  • Prof. Beat Meier

Printable Handouts

PDF

Navigable Slide Index

1. Introduction
2. Prions
3. What is [PSI+]?
4. Domain structure of the Sup35 (eRF3) protein
5. Suppression of the ade2 nonsense mutations
6. Nonsense suppression by Sup35 overproduction
7. [PSI+] curing by overproduced chaperones
8. Hsp104 chaperone level and [PSI+] curing
9. Effect of Hsp104 excess on Sup35
10. Polymerization-fragmentation model
11. [PSI+] prion particles contain Sup35 polymers
12. Hsp104 depletion affects size of Sup35 polymers
13. Effet of GuHCl on size of Sup35 polymers
14. [PSI+] and Hsp104: conclusions
15. Continuous Sup35 overproduction
16. Sup35 multicopy suppression and [PSI+]
17. Observations
18. Sup35 levels and nonsense codon readthrough
19. Continuous Sup35 overproduction: conclusions
20. PolyQ/QY proteins polymerization in [PIN+] cells
21. Differences between [PSI+] variants
22. Sup35 N domain
23. [PSI+] transmission from WT to mutant Sup35 (1)
24. Results of Sup35 PrD repeats deletion
25. [PSI+] transmission by truncated Sup35 mutants
26. [PSI+] transmission from WT to mutant Sup35 (2)
27. Co-expression of wild-type and mutant Sup35
28. Effect of the co-expression on polymers size
29. Prion fold and insertion of Sup35 WT/mutant
30. Sup35 truncations analysis: conclusions
31. Acknowledgements

Topics Covered

• Prion
• Yeast prions as non-chromosomal genetic determinants
• Modular structure of the Sup35 protein responsible for the yeast [PSI+] prion
• Role of the Hsp104 chaperone in [PSI+] prion propagation
• Two-level structure of the [PSI+] prion aggregates and methods for studying the size of prion polymers
• Non-prion amyloids in yeast
• Variability of the yeast [PSI+] prion

Links

Series:

• Prions and Amyloids

Categories:

• Biochemistry
• Cell Biology

Talk Citation

Publication History

• Published on September 4, 2008
• Archived on February 25, 2021

Financial Disclosures

• Prof. Michael Ter-Avanesyan has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.