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1. Introduction
2. Prions
3. What is [PSI+]?
4. Domain structure of the Sup35 (eRF3) protein
5. Suppression of the ade2 nonsense mutations
6. Nonsense suppression by Sup35 overproduction
7. [PSI+] curing by overproduced chaperones
8. Hsp104 chaperone level and [PSI+] curing
9. Effect of Hsp104 excess on Sup35
10. Polymerization-fragmentation model
11. [PSI+] prion particles contain Sup35 polymers
12. Hsp104 depletion affects size of Sup35 polymers
13. Effet of GuHCl on size of Sup35 polymers
14. [PSI+] and Hsp104: conclusions
15. Continuous Sup35 overproduction
16. Sup35 multicopy suppression and [PSI+]
17. Observations
18. Sup35 levels and nonsense codon readthrough
19. Continuous Sup35 overproduction: conclusions
20. PolyQ/QY proteins polymerization in [PIN+] cells
21. Differences between [PSI+] variants
22. Sup35 N domain
23. [PSI+] transmission from WT to mutant Sup35 (1)
24. Results of Sup35 PrD repeats deletion
25. [PSI+] transmission by truncated Sup35 mutants
26. [PSI+] transmission from WT to mutant Sup35 (2)
27. Co-expression of wild-type and mutant Sup35
28. Effect of the co-expression on polymers size
29. Prion fold and insertion of Sup35 WT/mutant
30. Sup35 truncations analysis: conclusions
31. Acknowledgements

Topics Covered

• Prion
• Yeast prions as non-chromosomal genetic determinants
• Modular structure of the Sup35 protein responsible for the yeast [PSI+] prion
• Role of the Hsp104 chaperone in [PSI+] prion propagation
• Two-level structure of the [PSI+] prion aggregates and methods for studying the size of prion polymers
• Non-prion amyloids in yeast
• Variability of the yeast [PSI+] prion

Links

Series:

• Prions and Amyloids

Categories:

• Biochemistry
• Cell Biology

Talk Citation

Ter-Avanesyan, M. (2008, September 4). Propagation and variability of the yeast [PSI+] prion [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 15, 2025, from https://doi.org/10.69645/UKYB5766.
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Publication History

• Published on September 4, 2008
• Archived on February 25, 2021

Financial Disclosures

• Prof. Michael Ter-Avanesyan has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.