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1. Introduction
2. Research goal
3. A specific protein
4. DNA recognition by the protein
5. Recognition of double stranded DNA
6. Polydactyl Zinc Finger protein
7. Talk outline
8. A diversity of recognition domains
9. The Zinc Finger domain
10. Zif268, a Cys2-His2 Zinc Finger protein
11. Key interactions of polydactyl Zinc Finger protein
12. Life in the major groove
13. A complete Zinc Finger code
14. Phage display
15. Phage display mechanism
16. Selection of the specific Zinc Finger protein
17. Results of phage selections
18. Molecular recognition in the GNN family of Zifs
19. Exquisite sequence specificity of Zif domains
20. Assembly of new Zinc Finger specific proteins (1)
21. Assembly of new Zinc Finger specific proteins (2)
22. 3-finger proteins are highly specific
23. Three fingers... 9 bp recognition
24. The specificity issue and genome size
25. 6-finger-ed specify a unique site in human genome
26. Genomic specificity requires polydactyl proteins
27. Non-additive linkage effects
28. Structure of the first 6-finger protein Aart
29. 6xGNN and 6xRNN sites in promoter sequences
30. Alignment of erbB-2 target seq. and erbB-3 5'-UTR
31. Co-expression of Zinc Finger proteins and GFP
32. Activation and repression domains
33. Specific regulation of two oncogenes
34. Affinities of E2C and E3 six finger proteins
35. Regulation of hemoglobin for sickle cell therapy
36. Efficient and specific gamma-globin regulation
37. Inhibition of HIV replication
38. Zinc Finger transcription factors in planta
39. Activation and repression in Arabidopsis
40. Creating cell and organism libraries
41. Transcription factors libraries
42. Every gene on or up, every gene off
43. TFZF selections in vivo
44. Cellular phenotypes induced by TF-ZF
45. TFZF promotes cell invasion in vitro and in vivo
46. Specific regulation of E48 antigen
47. Opportunities in synthetic biology
48. Employing libraries of Zif to screen for mutants
49. Chemical regulation of genes
50. Induction of angiogenesis in a mouse model
51. Additional actions of Zif proteins
52. Scissors for genome surgery
53. Integration, disruption and RMCE
54. Selected serine recombinase crystal structures
55. Recombinases: a powerful enzyme class
56. Zinc Finger - recombinase design
57. Zinc finger - recombinase evolution (1)
58. Zinc finger - recombinase evolution (2)
59. Selective recombination
60. Selective gene excision in human cells
61. Gene delivery vehicles
62. Targeted integration into ErbB2 locus
63. Designer recombinase applications
64. Epigenetic control
65. Reassembly of M.HhaI
66. In vivo site-specific DNA methylation
67. Targeted methylation
68. Sequence-enabled methylase and site-specificity
69. Site-specific DNA modification
70. Opportunities in nano-construction
71. Transcription factor and enzyme design automated
72. www.Zincfingertools.org (1)
73. Zinc Finger technology
74. Zinc Finger technology....or RNAi?
75. Acknowledgments
76. Our collaborators
77. Licensees of our Zif technology

Topics Covered

• Development of a universal system for gene regulation
• A diversity of recognition domains
• Phage display
• Targeted endogenous gene regulation in cells and organisms
• The specificity issue and genome size
• Genomic specificity requires polydactyl proteins
• Transcription factor libraries: a powerful new strategy
• Cutting and pasting genes and genomes
• Zinc finger enzymes and designer epigenetic modifications
• Zinc finger nucleases: scissors for genome surgery
• Recombinases
• Gene delivery vehicles

Links

Series:

• Chemical Biology

Categories:

• Biochemistry
• Cell Biology
• Genetics & Epigenetics
• Methods

Talk Citation

Barbas, C. (2008, August 14). Polydactyl zinc finger proteins: software and hardware for genomes [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 16, 2024, from https://hstalks.com/bs/942/.
Export Citation (RIS)

Publication History

• Published on August 14, 2008

Financial Disclosures

• Prof. Carlos Barbas has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.