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Navigable Slide Index

1. Introduction
2. Structure of the eye
3. Visual pigments
4. Rhodopsin structure (1)
5. Rhodopsin structure (2)
6. Why 11-cis-retinal?
7. Terpenoid biosynthesis
8. Visual chromophores
9. Bioluminescent squid
10. The bovine rhodopsin
11. Activation of Rh and beta AR
12. Point charge model
13. Retinal / vitamin A cycle
14. Selective capture of retinoid binding proteins
15. Photocrosslinking of chromophore to Rh
16. Tracking the chromophore/opsin interaction
17. Rhodopsin photocycle
18. Photoaffinity labeling: DK-Lumi, seq. temp. - 80 C
19. An HPLC profile
20. Summary of the binding a.a. (1)
21. Summary of the binding a.a. (2)
22. Conformation determination of 11-cis-retinal
23. Chromophore conformation in Rh
24. Transient "dark activity" of 13-des-Me-retinal
25. Retinoids that form pigments
26. Photoaffinity- rhodopsin-G protein interaction
27. Age-related macular degeneration (AMD)
28. Age pigments or RPE lipofuscin
29. A2E fluorophore
30. A2E FAB-MS w/wo irr. blue light, 450 nm, 10 min
31. UV/vis spectra of oxo-A2Es
32. Formation of A2E, iso A2E and ATR dimers
33. A2- rhodopsin
34. ABCR- ATP binding cassette protein
35. Bilberry extract and A2E epoxidation
36. Anthocyanins
37. Acknowledgements

Topics Covered

• Visual pigments
• Visual chromophores
• Activation of Rh and beta-AR
• Retinal / vitamin A cycle
• Selective capture of retinoid binding proteins
• Photocrosslinking of chromophore to Rh
• Tracking the chromophore / opsin interaction
• Rhodopsin photocycle
• Photoaffinity labelling
• Chromophore confirmation in Rh
• Transient 'dark activity' of 13-des-Me-retinal
• Retinoids that form pigments
• Photoaffinity study of rhodopsin: G-protein (transducin) interaction
• Age-related macular degeneration
• Age pigments or RPE lipofuscin
• A2E flurophore
• ATP binding cassette protein
• Bilberry extract vs. blue-light induced A2E epoxidation

Links

Series:

• Chemical Biology

Categories:

• Biochemistry
• Clinical Practice

Therapeutic Areas:

• Ophthalmology

Talk Citation

Publication History

• Published on August 14, 2008

Financial Disclosures

• Prof. Koji Nakanishi has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.