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1. Introduction
2. How are RNA machines assembled?
3. Components of the 70S ribosome
4. Prokaryotic RNA processing
5. Co-transcriptional assembly and RNA processing
6. Ribosome demand and bacterial growth
7. RNA vs. protein folding
8. RNA-protein complex formation
9. RNA folding in vivo
10. The assembly map
11. The electron density map
12. Domain structure of the 30S ribosome
13. Tertiary binding proteins assemble decoding site
14. A 30S assembly intermediate
15. 30S proteins binding kinetics and binding order
16. Understanding the mechanism of 30S assembly
17. 30S assembly map
18. A tale of two junctions
19. The minimal S15 binding site
20. A fluorescence assay for S15 binding
21. Single molecule fluorescence
22. Single molecule correlation spectroscopy
23. Analysis of Aquifex aeolicus S15:S6:S18 complex
24. Mg-independent RNA binding by Aquifex S15
25. Isothermal titration calorimetry of S6:S18 complex
26. Formation of S6:S18-S15-RNA complex
27. Thermodynamics of the complex formation
28. Binding of S6:S18 to RNA is dependent on S15
29. Crystal structure of the T4 RNA-protein complex
30. S15 contacts to helix 23a
31. Insights from detailed studies of the cental domain
32. Monitoring the 30S assembly reaction
33. Isotope pulse-chase assay to monitor assembly
34. High resolution MALDI-TOF analysis of TP-30
35. Representative progress curves at 40C degrees
36. Simultaneous monitoring of 30S protein binding
37. Assembly proceeds from 5' to 3'
38. Binding rates of 30S proteins
39. The temperature dependence of binding kinetics
40. 30S proteins binding kinetics: 15 vs. 40C degrees
41. Binding kinetics of post RI to RI* transition proteins
42. Arrhenius analysis of binding activation energy
43. Rates and activation energies are poorly correlated
44. The kinetic map and the 30S landscape
45. Summary
46. Acknowledgment

Topics Covered

• Bacterial ribosome biogenesis
• The Nomura assembly map
• RNA folding and protein binding
• Single molecule fluorescence
• The basis for cooperative assembly
• Isotope pulse chase experiments
• Protein binding kinetics
• The assembly landscape

Links

Series:

• The Mechanisms of Ribosome Function

Categories:

• Biochemistry
• Cell Biology

Talk Citation

Williamson, J. (2008, May 15). Assembly of the 30S ribosomal subunit in vitro and in cells [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 15, 2025, from https://doi.org/10.69645/NSPK4516.
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Publication History

• Published on May 15, 2008

Financial Disclosures

• Prof. James Williamson has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.