• Introduction and General Properties of Plasmids
• 31 min
  1. Introduction to plasmid biology

  • Prof. Emeritus Don Clewell
• 39 min
  2. General properties and applications of plasmids

  • Prof. Emeritus Don Clewell
• Replication
• 47 min
  3. Plasmids: copy number control by antisense RNAs

  • Prof. Gerhart Wagner
• 30 min
  4. Plasmid rolling-circle (RC) replication

  • Prof. Saleem Khan
• 45 min
  5. Replication of linear plasmids in bacteria

  • Prof. George Chaconas
• Maintenance and Partitioning
• 32 min
  6. Plasmid segregation and stability in bacteria

  • Prof. Barbara Funnell
• 33 min
  7. Plasmid stabilization by cell killing

  • Prof. Keith Weaver
• 30 min
  8. Dimer catastrophes and plasmid resolution

  • Dr. David Summers
• Conjugation and Mobilization
• 41 min
  9. The diversity of bacterial conjugation and mobilization systems

  • Prof. Fernando de la Cruz
• 36 min
  10. The pCF10-encoded pheromone response: cell-cell signaling, complexity and evolution

  • Prof. Gary Dunny
• 27 min
  11. DNA processing in conjugative transfer

  • Dr. Joel Schildbach
• 43 min
  12. Agrobacterium tumefaciens

  • Dr. Stephen Winans
• Genetic Traits Frequently Associated with Plasmids
• 37 min
  13. Insertion sequences and DNA transposition

  • Prof. William Reznikoff
• 48 min
  14. Plasmids, integrons and the spread of antibiotic resistance

  • Prof. Hatch Stokes
• 30 min
  15. Virulence plasmids

  • Prof. Julian Rood
• Eukaryotic Plasmids
• 30 min
  16. The yeast plasmid: a highly persistent selfish DNA element

  • Prof. Makkuni Jayaram
• Population Biology and Evolution
• 35 min
  17. Evolution of plasmids and their role in bacterial diversity and adaptability

  • Prof. Christopher Thomas
• Archived Lectures *These may not cover the latest advances in the field
• 85 min
  18. Bacterial extrachromosomal elements: role in chemical detoxification and potential cancer therapy

  • Prof. Ananda Chakrabarty
• 34 min
  19. Plasmid segregation and stability in bacteria

  • Prof. Barbara Funnell
• 39 min
  20. Replication of linear plasmids in bacteria

  • Prof. George Chaconas

Printable Handouts

PDF

Navigable Slide Index

1. Introduction
2. DNA plasmids
3. The yeast plasmid
4. Plasmid amplification
5. Fluorescence-tagged 2 micron plasmid
6. Plasmid dynamics during the cell cycle
7. Chromosome and plasmid segregation
8. Cell cycle and cohesin-STB association
9. Cohesin cleavage and plasmid partitioning
10. CSE4-STB association
11. Cohesin-STB association in the CSE4 mutant
12. Protein association at STB at G1-S
13. Pathway for equal plasmid segregation
14. Cohesion in the 2 micron plasmid
15. Plasmid pairing is cohesin-mediated
16. Cohesin-mediated sister-to-sister counting
17. Random grouping of replicated plasmids
18. STB-STB segregation
19. CEN-CEN segregation
20. CEN-STB segregation
21. Summary

Topics Covered

• The yeast plasmid as a highly optimized design for a multi-copy selfish DNA element
• The need for an active partitioning system for the segregation of a high copy plasmid
• Coupling between plasmid segregation and chromosome segregation
• Poaching of host factors required for chromosome segregation by the plasmid partitioning system
• Cohesin mediated equal segregation of duplicated plasmid clusters

Links

Series:

• Biology and Significance of Plasmids

Categories:

• Genetics & Epigenetics
• Methods
• Microbiology

Therapeutic Areas:

• Infectious Diseases

Talk Citation

Publication History

• Published on January 28, 2008

Financial Disclosures

• Prof. Makkuni Jayaram has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.