Registration for a live webinar on 'Precision medicine treatment for anticancer drug resistance' is now open.
See webinar details
This is a limited length demo talk; you may
login or
review methods of
obtaining more access.
- Introduction and General Properties of Plasmids
-
31 min
1. Introduction to plasmid biology- Prof. Emeritus Don Clewell
-
39 min
2. General properties and applications of plasmids- Prof. Emeritus Don Clewell
- Replication
-
47 min
3. Plasmids: copy number control by antisense RNAs- Prof. Gerhart Wagner
-
30 min
4. Plasmid rolling-circle (RC) replication- Prof. Saleem Khan
-
45 min
5. Replication of linear plasmids in bacteria- Prof. George Chaconas
- Maintenance and Partitioning
-
32 min
6. Plasmid segregation and stability in bacteria- Prof. Barbara Funnell
-
33 min
7. Plasmid stabilization by cell killing- Prof. Keith Weaver
-
30 min
8. Dimer catastrophes and plasmid resolution- Dr. David Summers
- Conjugation and Mobilization
-
41 min
9. The diversity of bacterial conjugation and mobilization systems- Prof. Fernando de la Cruz
-
36 min
-
27 min
11. DNA processing in conjugative transfer- Dr. Joel Schildbach
-
43 min
12. Agrobacterium tumefaciens- Dr. Stephen Winans
- Genetic Traits Frequently Associated with Plasmids
-
37 min
13. Insertion sequences and DNA transposition- Prof. William Reznikoff
-
48 min
14. Plasmids, integrons and the spread of antibiotic resistance- Prof. Hatch Stokes
-
30 min
15. Virulence plasmids- Prof. Julian Rood
- Eukaryotic Plasmids
-
30 min
16. The yeast plasmid: a highly persistent selfish DNA element- Prof. Makkuni Jayaram
- Population Biology and Evolution
-
35 min
17. Evolution of plasmids and their role in bacterial diversity and adaptability- Prof. Christopher Thomas
- Archived Lectures *These may not cover the latest advances in the field
-
85 min
-
34 min
19. Plasmid segregation and stability in bacteria- Prof. Barbara Funnell
-
39 min
20. Replication of linear plasmids in bacteria- Prof. George Chaconas
Printable Handouts
Navigable Slide Index
- Introduction
- Talk outline
- What are IS elements?
- Why are IS elements interesting?
- Discovery of insertion sequences (IS elements)
- Transposition
- IS structure
- Gamma-delta and Tn4
- IS elements can form composite transposons
- Composite transposon formation
- Cut and paste vs. replicative transposition
- Mechanism of cut and paste IS transposition
- IS50-like transposition
- Crystal structure of the IS50 synaptic complex
- Transposase proteins
- Regulation of IS transposition
- Role of IS elements in bacterial genetics
- Schematic of the F plasmid
- IS-mediated integration of F to generate HFR
- IS-mediated F' formation
- F' formation by deletion formation
- R factors
- Conclusion
Topics Covered
- Insertions sequences
- A major form of mobile genetic elements in eubacteria and archaebacteria
- Insertion sequences move via DNA transposition
- Insertion sequences cause genome changes (insertions, deletions, inversions and chromosome fusions) and facilitate the horizontal transfer of genes
- Related processes include integration of HIV-1 DNA
Links
Series:
Categories:
Talk Citation
Reznikoff, W. (2008, January 28). Insertion sequences and DNA transposition [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved December 26, 2024, from https://doi.org/10.69645/AIRB5151.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. William Reznikoff has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
Insertion sequences and DNA transposition
Published on January 28, 2008
37 min
