• Introduction and General Properties of Plasmids
• 31 min
  1. Introduction to plasmid biology

  • Prof. Emeritus Don Clewell
• 39 min
  2. General properties and applications of plasmids

  • Prof. Emeritus Don Clewell
• Replication
• 47 min
  3. Plasmids: copy number control by antisense RNAs

  • Prof. Gerhart Wagner
• 30 min
  4. Plasmid rolling-circle (RC) replication

  • Prof. Saleem Khan
• 45 min
  5. Replication of linear plasmids in bacteria

  • Prof. George Chaconas
• Maintenance and Partitioning
• 32 min
  6. Plasmid segregation and stability in bacteria

  • Prof. Barbara Funnell
• 33 min
  7. Plasmid stabilization by cell killing

  • Prof. Keith Weaver
• 30 min
  8. Dimer catastrophes and plasmid resolution

  • Dr. David Summers
• Conjugation and Mobilization
• 41 min
  9. The diversity of bacterial conjugation and mobilization systems

  • Prof. Fernando de la Cruz
• 36 min
  10. The pCF10-encoded pheromone response: cell-cell signaling, complexity and evolution

  • Prof. Gary Dunny
• 27 min
  11. DNA processing in conjugative transfer

  • Dr. Joel Schildbach
• 43 min
  12. Agrobacterium tumefaciens

  • Dr. Stephen Winans
• Genetic Traits Frequently Associated with Plasmids
• 37 min
  13. Insertion sequences and DNA transposition

  • Prof. William Reznikoff
• 48 min
  14. Plasmids, integrons and the spread of antibiotic resistance

  • Prof. Hatch Stokes
• 30 min
  15. Virulence plasmids

  • Prof. Julian Rood
• Eukaryotic Plasmids
• 30 min
  16. The yeast plasmid: a highly persistent selfish DNA element

  • Prof. Makkuni Jayaram
• Population Biology and Evolution
• 35 min
  17. Evolution of plasmids and their role in bacterial diversity and adaptability

  • Prof. Christopher Thomas
• Archived Lectures *These may not cover the latest advances in the field
• 85 min
  18. Bacterial extrachromosomal elements: role in chemical detoxification and potential cancer therapy

  • Prof. Ananda Chakrabarty
• 34 min
  19. Plasmid segregation and stability in bacteria

  • Prof. Barbara Funnell
• 39 min
  20. Replication of linear plasmids in bacteria

  • Prof. George Chaconas

Printable Handouts

PDF

Navigable Slide Index

1. Introduction
2. The anatomy of multicopy plasmids
3. Multicopy plasmid transmission during division
4. Metabolic load destabilises plasmids
5. Plasmid clustering may cause instability
6. Plasmid dimers and instability - dimer catastrophe
7. Distribution of plasmid dimers in culture
8. Recombination destabilises multicopy vectors
9. Plasmid multimer resolution
10. Converting dimers to monomers
11. The Xer-cer system of ColE1
12. The ColE1 cer nucleoprotein complex
13. Site inter-wrapping and recombination
14. Complex architecture and recombination
15. The gear shift-like recombination process
16. Simulation of the gear shift recombination
17. Supercoiling can facilitate recombination
18. Can recombination combat dimer catastrophe?
19. Preventing cell division before dimer resolution
20. Loss of Rcd checkpoint causes instability
21. Structure and function of Rcd
22. Identifying Rcd's target by affinity chromatography
23. Rcd stimulates indole production by tryptophanase
24. Multimers with cer sites elevate indole levels
25. E. coli exhibits a biphasic response to indole
26. Indole inhibits cell division by unknown mechanism
27. Summary
28. Further reading

Topics Covered

• Multicopy plasmid inheritance
• The causes of plasmid instability
• Plasmid multimer formation and the dimer catastrophe
• Plasmid dimer resolution by site-specific recombination
• The Rcd checkpoint and its role in multicopy plasmid stability

Links

Series:

• Biology and Significance of Plasmids

Categories:

• Genetics & Epigenetics
• Microbiology

Talk Citation

Publication History

• Published on January 28, 2008

Financial Disclosures

• Dr. David Summers has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.