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Printable Handouts
Navigable Slide Index
- Introduction
- The anatomy of multicopy plasmids
- Multicopy plasmid transmission during division
- Metabolic load destabilises plasmids
- Plasmid clustering may cause instability
- Plasmid dimers and instability - dimer catastrophe
- Distribution of plasmid dimers in culture
- Recombination destabilises multicopy vectors
- Plasmid multimer resolution
- Converting dimers to monomers
- The Xer-cer system of ColE1
- The ColE1 cer nucleoprotein complex
- Site inter-wrapping and recombination
- Complex architecture and recombination
- The gear shift-like recombination process
- Simulation of the gear shift recombination
- Supercoiling can facilitate recombination
- Can recombination combat dimer catastrophe?
- Preventing cell division before dimer resolution
- Loss of Rcd checkpoint causes instability
- Structure and function of Rcd
- Identifying Rcd's target by affinity chromatography
- Rcd stimulates indole production by tryptophanase
- Multimers with cer sites elevate indole levels
- E. coli exhibits a biphasic response to indole
- Indole inhibits cell division by unknown mechanism
- Summary
- Further reading
Topics Covered
- Multicopy plasmid inheritance
- The causes of plasmid instability
- Plasmid multimer formation and the dimer catastrophe
- Plasmid dimer resolution by site-specific recombination
- The Rcd checkpoint and its role in multicopy plasmid stability
Talk Citation
Summers, D. (2008, January 28). Dimer catastrophes and plasmid resolution [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved December 5, 2024, from https://doi.org/10.69645/EUUE1547.Export Citation (RIS)
Publication History
Financial Disclosures
- Dr. David Summers has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.