• Introduction and General Properties of Plasmids
• 31 min
  1. Introduction to plasmid biology

  • Prof. Emeritus Don Clewell
• 39 min
  2. General properties and applications of plasmids

  • Prof. Emeritus Don Clewell
• Replication
• 47 min
  3. Plasmids: copy number control by antisense RNAs

  • Prof. Gerhart Wagner
• 30 min
  4. Plasmid rolling-circle (RC) replication

  • Prof. Saleem Khan
• 45 min
  5. Replication of linear plasmids in bacteria

  • Prof. George Chaconas
• Maintenance and Partitioning
• 32 min
  6. Plasmid segregation and stability in bacteria

  • Prof. Barbara Funnell
• 33 min
  7. Plasmid stabilization by cell killing

  • Prof. Keith Weaver
• 30 min
  8. Dimer catastrophes and plasmid resolution

  • Dr. David Summers
• Conjugation and Mobilization
• 41 min
  9. The diversity of bacterial conjugation and mobilization systems

  • Prof. Fernando de la Cruz
• 36 min
  10. The pCF10-encoded pheromone response: cell-cell signaling, complexity and evolution

  • Prof. Gary Dunny
• 27 min
  11. DNA processing in conjugative transfer

  • Dr. Joel Schildbach
• 43 min
  12. Agrobacterium tumefaciens

  • Dr. Stephen Winans
• Genetic Traits Frequently Associated with Plasmids
• 37 min
  13. Insertion sequences and DNA transposition

  • Prof. William Reznikoff
• 48 min
  14. Plasmids, integrons and the spread of antibiotic resistance

  • Prof. Hatch Stokes
• 30 min
  15. Virulence plasmids

  • Prof. Julian Rood
• Eukaryotic Plasmids
• 30 min
  16. The yeast plasmid: a highly persistent selfish DNA element

  • Prof. Makkuni Jayaram
• Population Biology and Evolution
• 35 min
  17. Evolution of plasmids and their role in bacterial diversity and adaptability

  • Prof. Christopher Thomas
• Archived Lectures *These may not cover the latest advances in the field
• 85 min
  18. Bacterial extrachromosomal elements: role in chemical detoxification and potential cancer therapy

  • Prof. Ananda Chakrabarty
• 34 min
  19. Plasmid segregation and stability in bacteria

  • Prof. Barbara Funnell
• 39 min
  20. Replication of linear plasmids in bacteria

  • Prof. George Chaconas

Printable Handouts

PDF

Navigable Slide Index

1. Introduction
2. Preface
3. Terminology
4. Mechanism of action of PSK systems
5. Types of PSK
6. Typical proteic PSK
7. The Ccd system of plasmid F
8. The CcdA protein
9. CcdA-CcdB binding to DNA
10. Toxin-antitoxin structure
11. CcdB target: gyrase
12. GyrA binding of CcdB
13. Toxin-antitoxin families
14. Alternative arrangements of PSK systems
15. Modular organization of antitoxins
16. Chromosomally encoded proteic TA systems
17. Antisense-RNA regulated PSK loci
18. The problem with antisense RNA systems
19. The R1-encoded hok/sok locus
20. hok/sok solution to toxin mRNA persistence
21. Target of the Hok toxin: ghost cell formation
22. The pAD1 par locus
23. RNA I and RNA II secondary structures
24. Mechanism of RNA I-RNA II interaction
25. The RNA I-RNA II complex is unusually stable
26. Fst effects on chromosome segregation
27. Fst effects on cell division
28. Final note of caution
29. Review (general)
30. Review (proteic systems)
31. Review (antisense RNA regulated systems)

Topics Covered

• Introduction to post-segregational killing systems
• Proteic post-segregational killing systems
• The Ccd system
• Other proteic systems
• Antisense RNA-regulated post-segregational killing systems
• The plasmid R1 hok/sok system
• The plasmid pAD1 par system

Links

Series:

• Biology and Significance of Plasmids

Categories:

• Genetics & Epigenetics
• Microbiology

Therapeutic Areas:

• Infectious Diseases

Talk Citation

Publication History

• Published on January 28, 2008

Financial Disclosures

• Prof. Keith Weaver has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.