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Navigable Slide Index

1. Crystal structures of P450s
2. Structures of P450s
3. CYPs 3A4, 2A6 and 101
4. CYP2C5/diclofenac complex
5. Targeting of P450s to membranes
6. Microsomal P450s for crystall.
7. P450-membrane interactions
8. Proximal face of P450 2C5
9. Role of heme in O2 reduction
10. P450 heme binding site: CYP101 (1)
11. P450 heme binding site: CYP101 (2)
12. Substrate binding in CYP2C5
13. Family 2 P450s
14. P450s 2C8, 2C5 and 2A6
15. Comparison of CYP2C8/CYP2C5
16. Active site cavities - size/shape diff.
17. Active site shape and volume
18. Size discrimination
19. CYP2C5 - DFC vs. DMZ binding
20. DMZ/DFC complexes of CYP2C5
21. CYP2B4 - open/closed confo. (1)
22. CYP2B4 - open/closed confo. (2)
23. P450 flexibility
24. CYP2A6
25. Coumarin bound to CYP2A6
26. Substrate binding to P450s
27. CYP2C9 substrates
28. WT/mutant CYP2C9 conformations
29. CYP2C9 complexes - 1OG5/1R9O
30. Flurbiprofen contacts in CYP2C9
31. S-warfarin binding to CYP2C9
32. Small substrates - large active sites
33. Flurbiprofen complex of CYP2C9
34. CYP3A4 substrates
35. Structures of CYP 3A4 and 2C8
36. In silico docking of testosterone
37. CYP3A4 structures
38. Metyrapone bound to CYP3A4
39. Interfaces of CYP 2C8 and 3A4
40. CYP2C8 substrates
41. Montelukast bound to CYP2C8
42. Summary

Topics Covered

• Introduction to CYP450 architecture
• Shared structural features
• Catalytic mechanism
• Redox partner interactions
• Membrane topology
• Multiple modes of substrate binding and oxidation

Links

Series:

• Drug Metabolizing Enzymes

Categories:

• Biochemistry
• Metabolism & Nutrition
• Pharmaceutical Sciences

Therapeutic Areas:

• Cardiovascular & Metabolic

Talk Citation

Johnson, E. (2007, October 1). Crystal structures of drug-metabolizing P450 monooxygenases [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 15, 2025, from https://doi.org/10.69645/IRAM9877.
Export Citation (RIS)

Publication History

• Published on October 1, 2007
• Archived on November 17, 2016

Financial Disclosures

• Prof. Eric Johnson has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.