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Printable Handouts
Navigable Slide Index
- Introduction
- RNA interference (RNAi)
- RNAi: Post-Transcriptional Gene Silencing (PTGS)
- RNAi: Transcriptional Gene Silencing (TGS)
- dsRNA mediated TGS in S. Pombe
- Do siRNAs directed to promoters induce TGS?
- FIV lentiviral vector system
- Experimental protocol
- siRNA targeting EF1a promoter suppress GFP
- Specificity of EF1A siRNA Mediated TGS
- siRNA does not alter vector integration
- siRNA mediated TGS is reversible
- TGS is associated with DNA methylation
- siRNA EF52 can target the EF1A promoter
- EF1a is only inhibited at 48hrs post-transfection
- Are intronic regions susceptible to siRNA targeting
- Intronic region is susceptible to siRNA targeting
- Specificity of EF315 transcriptional silencing
- siRNA mediated TGS in human cells
- What is the mechanism of siRNA mediated TGS?
- TGS operates via chromatin remodeling pathway
- siRNA mediated histone methylation
- RNA pol II is required for siRNA mediated TGS
- How do siRNAs find the target?
- Detection of flag-tagged DNMTs or Hp1s
- Specificity of DNMT binding
- Antisense siRNA targeting HIV-1 LTR/promoter
- Susceptibility of the HIV-1 LTR to siRNA
- siRNA EF52 and all the factors are linked
- Conclusions
- Putative mechanism(s) for siRNA mediated TGS
- Acknowledgements
- Living on the edge
Topics Covered
- How siRNAs mediate transcriptional gene silencing (TGS) in human cells
- Histone methylation
- Dependency on active RNA polymerase II mediated transcription
- siRNA-mediated TGS
- H3K9 and H3K27 methylation of the targeted EF1A promoter
- Nuclear specific delivery of the siRNA
- Antisense strand-specific siRNA-mediated TGS of EF1A
- Targeting of the U3 region of the HIV-1 LTR/promoter
- Antisense strand of siRNA EF2 interacts with FLAG-tagged DNMT3A and the tri-methylated H3K27 methyl mark
- siRNA mediated targeting of genomic regions
- RNA Pol-II function
- Transcriptional gene silencing in human cells
Talk Citation
Morris, K. (2007, October 1). Small interfering RNA induced transcriptional gene silencing in human cells [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved October 4, 2024, from https://doi.org/10.69645/VHCZ2546.Export Citation (RIS)
Publication History
Financial Disclosures
- Dr. Kevin Morris has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
Small interfering RNA induced transcriptional gene silencing in human cells
A selection of talks on Genetics & Epigenetics
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