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Navigable Slide Index

1. Introduction
2. Topics: definitions and background
3. Definition of genomics and functional genomics
4. Definition of proteomics and functional proteomics
5. Levels of gene expression
6. Systems biology
7. Themes in microarray technology
8. Genomics microarray technology
9. Genomics microarray technology - definitions
10. Topics: how to do microarrays
11. Three types of genomics microarray platforms
12. Probes and targets
13. A DNA microarray
14. Microarray printer
15. Microarray printing pins
16. Slide substrate chemistry: amine silane
17. Target labeling
18. Summary: two-probe hybridization
19. Microarray slide scanner/Gridder
20. Mouse microarray
21. Human microarray
22. Scatter plot
23. Integrated gene-expression databases
24. Affymetrix microarrays
25. Microarray research applications
26. Microarray publication rate
27. Microarray applications
28. Example application: aryl hydrocarbon receptor
29. Levels of RNA expression
30. An experimental design
31. Other applications for DNA microarrays
32. Future genomics technologies
33. Lynx beads
34. Illumina fiber optics
35. 3M heat shrinkage technology
36. Nanogen DNA chip
37. Proteomics - microarray technology
38. Proteomics - definitions and background
39. Proteome - definition
40. Protein levels vs. mRNA levels
41. Protein acitivity levels vs. mRNA levels
42. Ways to study the proteome
43. Challenges for protein microarrays
44. How to do protein microarrays
45. Two major types of protein microarrays
46. Two types of protein microarrays - function based
47. Constructing protein microarrays
48. Printing substrates for protein microarrays
49. Detection methods for protein microarrays
50. Advantages/disadvantages of detection methods
51. Research applications for protein microarrays
52. Applications for abundance-based arrays
53. Applications for function-based arrays
54. Future of protein microarray technology
55. Proteomics here to stay

Topics Covered

• High-throughput methods to examine global gene expression
• Understanding interactions in a given cell, tissue and organism under normal and disease state conditions
• Application of high throughput methods used in genomics and proteomics
• Use of DNA and protein microarrays for gene discovery and function
• Background information and terminology used in genomics and proteomics
• How DNA and protein microarrays are carried out
• Predicted future direction of genomics and proteomics microarray technologies

Links

Series:

• From DNA to Proteins

Categories:

• Cell Biology
• Genetics & Epigenetics
• Methods

Talk Citation

Tomlinson, C.R. (2007, October 1). Gene and protein microarrays: tools for gene discovery and function [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 15, 2025, from https://doi.org/10.69645/ZTHR6219.
Export Citation (RIS)

Publication History

• Published on October 1, 2007
• Archived on August 13, 2020

Financial Disclosures

• Prof. Craig R. Tomlinson has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.