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- Introduction
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1. From DNA to proteins: the multiple levels of regulation
- Prof. Panagiotis Tsonis
- Chromatin and DNA
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2. Chromatin architecture and alterations in the control of gene regulation
- Prof. Jeffrey Hansen
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3. Nucleosome dynamics and remodeling
- Prof. Jonathan Widom
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4. DNA replication
- Prof. Smita Patel
- Transcription
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6. Mechanisms of transcription: the eukaryotic pre-initiation complex
- Prof. Michael Hampsey
- RNA (I)
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7. Pre-mRNA splicing
- Prof. Timothy Nilsen
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8. Self-splicing intron RNAs: ribozymes, parasites and agents of genomic change
- Prof. Anna Marie Pyle
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10. mRNA capping
- Prof. Aaron Shatkin
- RNA (II)
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13. RNA editing: changing the code in plants, animals and parasites
- Prof. Stephen Hajduk
- Proteins
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14. The principle of translation
- Prof. Joachim Frank
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15. Translation initiation
- Prof. Patrick Linder
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16. Elongation of protein synthesis: structural basis of the process of decoding
- Prof. Marina Rodnina
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17. Elongation of protein synthesis: structural basis of the peptide bond
- Prof. Marina Rodnina
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18. How do proteins fold and why?
- Prof. Walter Englander
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19. Protein degradation
- Prof. Alfred Goldberg
- Understanding Global Gene Expression
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21. Comparing transcriptomes of distant organisms: models of gene expression 2
- Prof. Mark Gerstein
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22. Protein networks and analysis of global gene expression
- Dr. Trey Ideker
- Archived Lectures *These may not cover the latest advances in the field
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23. Chromatin and transcription
- Prof. Roger Kornberg
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24. Nuclear organization and gene expression
- Prof. David Spector
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25. Gene and protein microarrays: tools for gene discovery and function
- Prof. Craig R. Tomlinson
Printable Handouts
Navigable Slide Index
- Introduction
- mRNA 5' cap structure
- mRNA cap functions
- Cap binding and translation initiation
- Cap synthesis
- Capping enzyme structure
- Complementation of S.cerevisiae lacking GT
- Recruitment of mRNA cap enzymes by P-CTD
- P-CTD enhances guanylylation and cap formation
- hSPT5 is a transcription elongation factor
- SPT5 enhances guanylylation and capping
- DSIF overcome FCP1-mediated capping decrease
- Capping enzyme reverses repression by NELF
- Importin-alfa
- Imp-alfa increases MT activity
- MT selectively binds GpppG-RNA
- Imp-alfa enhances MT targeting to GpppG-RNA
- Importation of nuclear proteins
- Imp-beta blocks Imp/MT/RNA formation
- RanGTP counteracts Imp-beta effects
- Capping modulation
- MT regions required for Imp-alfa binding
- MT sequence localizes proteins to nucleus
- MT NLS narrowed
- Alternative NLSs in MT
- Nuclear localized MT binds pol II
- Capping enzyme RNAi is lethal in C.elegans
- SiRNA inhibits expression of capping enzymes
- Apoptosis - cleavage of caspase-3
- RNA interference induces apoptosis
- Inducible CE RNAi causes apoptosis
- Summary
- Thanks
Topics Covered
- Modification of mRNA 5' ends by addition of a m7G cap structure
- Capping for viability
- Conservation in yeast to humans
- Structure of caps and capping enzymes
- Mechanism of cap synthesis
- Protein-protein interactions that link capping to events in transcription, transport and translation
- siRNA knockdown experiments
Talk Citation
Shatkin, A. (2007, October 1). mRNA capping [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 1, 2025, from https://doi.org/10.69645/RMOR3353.Export Citation (RIS)
Publication History
- Published on October 1, 2007
Financial Disclosures
- Prof. Aaron Shatkin has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.