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1. Introduction
2. Advantages of chloroplast transformation
3. Tobacco chloroplast DNA
4. Introducing DNA into plant cell
5. Selection process
6. Transgene integration and homoplasmy
7. Transgene expression
8. Maternal inheritance: no pollen transmission
9. Maternal inheritance and gene containment
10. An introduction of a herbicide resisting gene
11. Creating male sterile plants
12. Reversibility of male sterility
13. Molecular strategies for gene containment
14. Bt cry2Aa2 operon overexpression in chloroplasts
15. Chloroplasts containing Cry: electron micrographs
16. The highest level of foreign gene expression
17. High levels of expression - high insecticidal activity
18. E. coli expression of (GVGVP)121
19. pHBA expression in transgenic chloroplasts
20. CPL transgenic plants 4B highest pHBA levels
21. Phytoremediation of organomercurial compounds
22. Hg accumulation in roots and shoots of tobacco
23. Phytoremediation via the chloroplast genome
24. Trehalose accumulation confers drought tolerance
25. TPS plants grow in the presence of PEG
26. Accumulation of trehalose in the nucleus is toxic
27. Chloroplast genetic engineering
28. The potent protein MSI 99
29. MSI 99, expressed via the plastid genome
30. Carrot plastid transformation (1)
31. Cotton plastid transformation
32. Soybean plastid transformation
33. Plastid transformation and genes transmission
34. Agronomic traits in chloroplast genetic engineering
35. Tobacco vs. E. coli
36. Gene regulation in transgenic chloroplasts
37. Recombinant human serum albumin
38. Electron micrographs of HSA in chloroplasts
39. Field production of chloroplast-derived IFN-alpha 2b
40. Transgenic plants grown in the field
41. Chloroplast-derived IFN-alpha 2b ELISA - T1 LAMD
42. Chloroplast-derived IFN-alpha 2b ELISA summary
43. VSV CPE assay (1)
44. VSV CPE assay (2)
45. Purified IFN-alpha 2b
46. In vitro conclusions
47. HIV luciferase assay (1)
48. HIV luciferase assay (2)
49. In vivo experimental protocol
50. Immunohistochemistry (1)
51. Immunohistochemistry (2)
52. Immunophenotypes
53. Proteins in the chloroplast genetic engineering
54. Immunogenic properties in chloroplast
55. Symptoms of anthrax disease
56. Qantification of chloroplast derived PA
57. PA expressed in pLD-VK1 tobacco transgenic
58. Macrophage lysis functional assay
59. Immunization studies of chloroplast derived PA
60. Toxin neutrolization assay
61. Toxin challenge
62. Chloroplast derived anthrax vaccine
63. Highly-expressed viral peptide animal vaccine
64. Vaccine Ag expressed via chloroplast engineering
65. Carrot plastid transformation (2)
66. Cholera toxin B delivery into the circulatory system
67. GFP expression seen under UV light
68. Mice intestine, liver and spleen cryosections
69. Confocal microscopic pictures of the liver
70. Cryo-sections of mouse liver
71. CTB-GFP immunostaining of various tissues
72. Carrot plastid transformation (3)
73. Cholera toxin B expression in chloroplasts
74. Tolerance induction by oral antigens
75. The lettuce plastid transformation system
76. A fully grown lettuce transgenic plant (1)
77. A fully grown lettuce transgenic plant (2)
78. Western blot analysis of human proinsulin
79. Results of an oral delivery of CTB- proinsulin
80. Verifying the reproducibility of the results
81. Insulitis score
82. Apoptotic pathway
83. Insulin immunoreactivity in mice fed with CTB-Pins
84. M cells, APCs, Th2 and cytokines interaction
85. IL-10 immunoreactivity in the pancreas
86. IL-4 immunoreactivity in the pancreas
87. Prevalence and incidence of autoimmune diseases
88. Extending the CROP cp genomes sequencing
89. The multipipmaker program
90. The reputer program
91. Sequence alignment of IR loss region
92. Cotton chloroplast genome analysis (1)
93. Cotton chloroplast genome analysis (2)
94. Cotton chloroplast genome analysis (3)
95. Comparison solanaceae chloroplast genomes ISRs
96. Grape chloroplast genome analysis
97. Recent review articles
98. Molecular biology of plant organellss

Topics Covered

• Advantages, concepts and methodology
• Maternal inheritance and cytoplasmic male sterility
• Agronomic traits to confer insect, herbicide, disease resistance, drought and salt tolerance and phytoremediation
• Transformed crop species
• Production and functional evaluation of human blood proteins (interferon) and vaccines (anthrax)
• Mechanism of oral delivery and protection against insulitis in non-obese diabetic mice
• Crop chloroplast genomics

Links

Series:

• Chloroplast

Categories:

• Biochemistry
• Cell Biology
• Plant & Animal Sciences

Talk Citation

Publication History

• Published on October 1, 2007

Financial Disclosures

• Prof. Henry Daniell has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.