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The chloroplast proteome.
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In this presentation,
I will review five subjects.
What is proteomics and
what does it measure?
Protein identification
by mass spectrometry,
including intact proteins, peptide
mass fingerprinting and sequence tags.
The problem of complex mixtures
and protein identification.
Quantitative proteomics and
stable isotope labeling,
intact protein mass measurement
and 'top-down' proteomics.
Let's start off by considering
what is proteomics and
what does it measure?
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Proteomics is a component
of systems biology.
"We would like a computer model
that mimics life in silico
allowing accurate projections for
metabolic engineering experiments."
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What exactly is proteomics?
Proteomics is the science
of describing the
complete set of proteins
expressed from an organism's
genetic material,
their interactions
with each other
and the influence
of development,
environment and disease
upon their expression.
Since understanding their
structure and function of
the gene products and their
interrelationships are the overall goals,
we can regard proteomics
alongside structural genomics
as a component of functional
genomics and systems biology.
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The cellular proteome
is a dynamic beast
continuously expressed
from the genome
under the influence of the
environment and nutrient conditions.
The proteome impinges back upon
the genome and back upon itself
to accomplish short-term
acclimation over minutes,
medium-term acclimation
over hours and days,
often involving changes
in gene expression,
and ultimately accomplishing
development of growth,
reproduction and evolution.
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We can consider proteome flux,
the flow of nutrients and
energy through transcription,
translation and
protein degradation
that is controlled
by the genetic code
and numerous factors that
influence expression.
Ideally, we would like to
monitor global gene expression,
protein post-translational
modification and turnover
with molecular resolution and
non-invasive technologies.
