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- Origins, Diversity and Persistence
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1. Plastid structure: development and differentiation
- Dr. Kevin Pyke
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2. Origin and molecular evolution of the plastid
- Prof. Debashish Bhattacharya
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3. The chloroplast genome and chloroplast gene expression
- Prof. Christopher Howe
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4. Regulation of chloroplast gene expression: light activated translation
- Prof. Stephen Mayfield
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5. Protein synthesis in plastids
- Dr. William Zerges
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6. The chloroplast proteome
- Dr. Julian Whitelegge
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7. Protein import into chloroplasts
- Dr. Ute Vothknecht
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8. Assembly of the photosynthesis apparatus
- Prof. J. Kenneth Hoober
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9. Plastid senescence
- Prof. Howard Thomas
- Biosynthetic Support of the Biosphere
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10. Photosynthesis: energy capture
- Prof. Robert Blankenship
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11. Photosynthesis: carbon assimilation
- Prof. Richard Leegood
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12. Regulation of photosynthetic carbon metabolism
- Prof. Louise Anderson
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13. Pigments: chlorophyll and its synthesis
- Prof. Wolfhart Rudiger
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14. Carotenoid functions and other isoprenoids in plants
- Prof. Elisabeth Gantt
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15. Chloroplast lipid biosynthesis
- Prof. Christoph Benning
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16. Amino acid synthesis in chloroplasts
- Prof. Peter Lea
- Prof. Paulo Mazzafera
- Dr. Ricardo Azevedo
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17. Responses of chloroplasts to stress conditions
- Dr. Ron Mittler
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18. Chloroplast genetic engineering
- Prof. Henry Daniell
- Archived Lectures *These may not cover the latest advances in the field
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19. The chloroplast genome and chloroplast gene expression
- Prof. Christopher Howe
Printable Handouts
Navigable Slide Index
- Introduction
- Talk outline
- First experimental observations
- Mirabilis crossing experiments
- Explanations for non-Mendelian inheritance
- Chlamydomonas mating experiments
- Evidence for genetic determinants in chloroplasts
- Identifying and mapping chloroplast genes
- First complete chloroplast genome sequence
- Organisms that retain a chloroplast
- The chloroplast genome
- Chloroplast DNA structure
- Core protein genes in chloroplast DNAs (1)
- Other chloroplast genes
- Remnant chloroplast genomes
- Core protein genes in chloroplast DNAs (2)
- Organization of a typical dinoflagellate minicircle
- Gene expression in model systems
- Transcription in vitro from trnM2 promoter
- Evidence for nuclear-encoded RNA polymerase
- Transcripts in lines disrupted for chloroplast genes
- Nuclear-encoded polymerase (NEP)
- Class I NEP promoters
- Post-transcriptional modification - splicing
- Trans-splicing
- Trans-splicing of psaA in Chlamydomonas
- Post-transcriptional modification - editing
- Tabacco psbL model system
- Post-transcriptional modification - editing
- Post-transcriptional modification - RNA turnover
- Chlamydomonas atpB
- Spinach petD
- Cleavage and exonuclease activity
- Post-transcriptional modification - RNA turnover
- Translation
- Why retain a chloroplast genome?
- Requirements for functional transfer to the nucleus
- Why retain nuclear copy?
- Why are any genes retained in the chloroplast?
- Chloroplast/nucleus signalling
- Why are any genes retained in the chloroplast?
- Summary
- References
Topics Covered
- Early evidence for a chloroplast genome
- Organization of the chloroplast genome in plants and algae
- Gene content
- Transcription
- Roles of bacterial and phage-type RNA polymerases
- Post-transcriptional modification
- Splicing, editing and RNA turnover
- Translation
- Genome evolution
- Transfer of genes to the nucleus
Talk Citation
Howe, C. (2007, October 1). The chloroplast genome and chloroplast gene expression [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved February 5, 2025, from https://doi.org/10.69645/BAUT8611.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Christopher Howe has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.