• Introduction to Protein Structure and Function
• 40 min
  1. Nature’s strategies in the regulation of enzyme activity by modifiers

  • Prof. Antonio Baici
• Creation of Protein Variability by Manipulation of Genes
• 28 min
  2. Understanding protein variability through functional analysis of random mutagenesis

  • Prof. Andrew Kaplan
• 48 min
  3. Perspectives on biological catalysis

  • Prof. Stephen Benkovic
• 45 min
  4. Fundamentals and principles for engineering proteolytic activity

  • Prof. Charles Craik
• Metabolic Diseases Caused by Genetic Mutation
• 36 min
  5. Modifications of pyruvate handling in health and disease

  • Prof. Mary Sugden
• 29 min
  6. Mitochondrial fatty acid oxidation deficiencies

  • Prof. Niels Gregersen
• 33 min
  7. Inborn errors of ketone body metabolism

  • Prof. Toshiyuki Fukao
• 34 min
  8. Cathepsin K in bone and joint diseases

  • Prof. Dieter Bromme
• 22 min
  9. Fabry disease: alfa-galactosidase A deficiency and enzyme replacement therapy

  • Prof. David Warnock
• 47 min
  10. Acid beta-glucosidase/glucocerebrosidase (GCase)

  • Prof. Gregory Grabowski
• 23 min
  11. GM2 gangliosidosis future treatments 1

  • Prof. Brian Mark
• 19 min
  12. GM2 gangliosidosis future treatments 2

  • Prof. Brian Mark
• 29 min
  13. The neuronal ceroid lipofuscinoses

  • Prof. Sandra Hofmann
• Disorders of Blood Coagulation
• 24 min
  14. Advances in fibrinolysis

  • Dr. Paul Kim
• 26 min
  15. Tissue factor and factor VII: initiation of blood coagulation

  • Dr. John McVey
• 36 min
  16. Structure of thrombin, a Janus-headed proteinase

  • Prof. Wolfram Bode
• 45 min
  17. The protein c-thrombomodulin mechanism: regulating multiple biological systems

  • Prof. Edward Conway
• 40 min
  18. Fibrinogen and factor XIII

  • Prof. John Weisel
• 31 min
  19. Factor VIII and haemophilia A

  • Dr. Geoffrey Kemball-Cook
• 34 min
  20. Factor IX

  • Prof. Bruce Furie
• 42 min
  21. The biology and pathobiology of von Willebrand factor

  • Prof. David Lillicrap
• 40 min
  22. Thrombotic thrombocytopenic purpura

  • Prof. J. Evan Sadler
• 26 min
  23. Fibrinolysis

  • Prof. Edward Tuddenham
• Other Molecular and Metabolic Disorders
• 45 min
  24. Glucose-6-phosphate dehydrogenase deficiency

  • Dr. Jane Leopold
• 26 min
  25. Cytochrome b5 reductase deficiency and hereditary methemoglobinemia

  • Dr. Scott Reading
• 50 min
  26. Sickle cell disease

  • Prof. Martin H. Steinberg
• 40 min
  27. Pyruvate kinase deficiency

  • Prof. Alberto Zanella
• 47 min
  28. Heritable disorders of collagen

  • Dr. Heather Yeowell
• 40 min
  29. Duchenne muscular dystrophy

  • Prof. Jeff Chamberlain
• Archived Lectures *These may not cover the latest advances in the field
• 33 min
  30. Protein crystallography

  • Prof. Michael James
• 48 min
  31. Regulation of blood coagulation by the serpin, antithrombin

  • Prof. Steve Olson
• 37 min
  32. Rhodopsin and retinitis pigmentosa

  • Dr. Shalesh Kaushal
• 40 min
  33. The physiology and pathology of coagulation factor XI

  • Dr. David Gailani
• 24 min
  34. Cytochrome b5 reductase deficiency and hereditary methemoglobinemia

  • Prof. Josef Prchal
• 49 min
  35. Metachromatic leukodystrophy

  • Prof. Volkmar Gieselmann
• 21 min
  36. Serpins and serpinopathies

  • Dr. James Whisstock
• 27 min
  37. Multiple acyl-CoA dehydrogenation deficiency: defects of electron transfer flavoproteins

  • Dr. Rikke Olsen
• 39 min
  38. Pleiotropic and epistatic genes in sickle cell anaemia

  • Prof. Ronald Nagel
• 44 min
  39. Genetic disorders of carbonic anhydrases II and IV

  • Prof. William Sly
• 39 min
  40. GM2 gangliosidoses

  • Prof. Don Mahuran
• 48 min
  41. Kinetic analysis of protein activity

  • Prof. Antonio Baici

Printable Handouts

PDF

Navigable Slide Index

1. Introduction
2. Content
3. Protease and inhibitor activities
4. Known proteases in the human genome
5. Human thiol-dependent cathepsins
6. The term "cathepsin"
7. Cathepsin K structure, expression and activity
8. Cathepsin K structure
9. Cathepsin K domain structure
10. Cathepsin K tissue distribution
11. Cathepsin K substrate specificity
12. Inhibition of cathepsin K
13. Cleavage of type I collagen by cathepsin K
14. What makes cathepsin K a unique collagenase?
15. Cathepsin K hydrolyses triple helical collagen
16. Activity of cathepsin K
17. Effect of chondroitin sulfate (CS)
18. Cathepsin K/CS complex identification
19. Cathepsin K/chondroitin complex formation
20. Collagenolytic activity and complex formation
21. Evidence that cathepsin K complexes exist in vivo
22. Pycnodysostosis
23. Pycno mutant Y212C has no collagenase activity
24. Pycno mutant Y212C does not form complexes
25. Collagens degradation by MMPs and cathepsins
26. Cathepsin K in osteoporosis
27. The human skeleton
28. Osteoclast activity
29. Cathepsin K and its expression in osteoclasts
30. Osteoporosis
31. Bone resorption assay (pit assay)
32. Effect of inhibitors of cathepsins K and L
33. Ratio of urine NTx/creatinine
34. Cathepsin K and osteoporosis (summary)
35. Cathepsin K in arthritis
36. Arthritis
37. Rheumatoid arthritis (RA)
38. Cell types involved in cartilage and bone erosion
39. Major bone and cartilage matrix proteins
40. Cathepsin K expression in the inflamed synovium
41. Expression of cathepsin K in the RA synovium
42. Cartilage erosion by CATK+ cells
43. Synovial cathepsin K expression and HFCQ
44. Scanning EM of MMP/cathepsin-digested cartilage
45. Inflammatory reaction involvement of cathepsin K
46. MHC class II antigen presentation
47. Expression of cathepsins K and S
48. Cathepsin K deficiency and CIA
49. Collagen-induced arthritis and cathepsins
50. Type II CIA (experimental model)
51. Ankle thickness of CIA
52. Diminished susceptibility to CIA
53. Bone erosion and cartilage damage
54. Cartilage degradation
55. Bone erosion
56. Potential cathepsin inhibitor therapy in RA
57. Conclusions

Topics Covered

• Cysteine proteases
• Cathepsin family
• Expression of cathepsins and their activity
• Collagen degradation
• Pycnodysostosis
• Cathepsin K in osteoporosis
• Cathepsin K in arthritis
• Mouse model of arthritis

Links

Series:

• Protein Epidemiology

Categories:

• Biochemistry
• Cell Biology
• Clinical Practice

Talk Citation

Publication History

• Published on October 1, 2007

Financial Disclosures

• Prof. Dieter Bromme has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.