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Hello, I'm Egidijius Machtejevas,
I'm the Lead Expert in Chromatography Products at Merck.
Today I would like to talk about the advantages of
using monolithic silica gel columns in the pharma industry.
I would also like to mention that this is part 1.
In part 1, I will talk about small molecular weight compounds,
and in part 2, I will focus on the larger molecular weight compound separations.
First I will start by introducing you to the world of monolithic silica.
Talking about chromatographic base materials, we have several different options.
We could use fully porous silica particles,
we could use fused-core superficially porous particles,
there are also fully porous polymeric particles, and also monolithic silicas.
All these different materials have certain strengths, and some weaknesses too.
For example, fully porous silicon materials have the best loadability.
Fused-core type of materials have the highest efficiency possible on the market.
In this presentation, I will focus on monolithic silica.
I will show in the slides to come, why monolithic silica can supply
certain advantages, and how best to benefit from using monolithic silica columns.
In HPLC method development, there are several important parts.
First of all we have to define our separation goals,
we need to know why we're separating and what the separation should look like.
Then of course, there is a lot of focus around the sample.
Sample is a key element, we have to know all the sample components,
impurities, concentration levels, sizes and so on.
I'll go more into details in my next slides.
Then if we are free to choose any instrument we could select one or another instrument.
It could be HPLC, it could be UHPLC, it depends which one you prefer, or what advantages you would like to get.
Then we select the column-based material, column modification,
we go to conditions, and the circle is closed.
We can verify how this aligns with our separation goals.