Design and Engineering of Zinc-Finger Domains

Published on October 1, 2007 Updated on April 30, 2017   44 min

Other Talks in the Series: Protein Folding, Aggregation and Design

In the last couple of decades, there has been a tremendous amount of progress in the design and engineering of a class of protein domain known as zinc fingers. This updated presentation is designed to give you an overview of the field.
I'll be dividing this talk into five parts. First of all, I'll give you an introduction to naturally occurring zinc fingers, what makes a protein domain a zinc finger, and some of the different types and functions of zinc fingers to give you an idea of the variety that already exists in nature. Then I'll go on to describe some de novo work that is being carried out using zinc fingers, an engineering strategy that have employed to graft desirable features of zinc fingers onto other protein domains. This will be followed up by some basic background on natural zinc finger DNA interactions. That's a lead-up into what has become one of the real success stories in protein design and engineering, which is the designing or engineering of specific DNA binders using polydactyl or poly-zinc finger approaches. I will then finish it up with some recent progress in the development of specific protein-binding zinc fingers to hopefully show you the potential of this small versatile protein domain as protein-binding targets and scaffolds.