In the last couple of decades,
there has been a tremendous amount
of progress in the design
and engineering of a class
of protein domain known as zinc fingers.
This updated presentation is designed
to give you an overview of the field.
I'll be dividing this talk
into five parts.
First of all,
I'll give you an introduction
to naturally occurring zinc fingers,
what makes a protein domain
a zinc finger,
and some of the different types
and functions of zinc fingers
to give you an idea of the variety
that already exists in nature.
Then I'll go
on to describe some de novo work
that is being carried out
using zinc fingers,
an engineering strategy
that have employed to graft
desirable features of zinc fingers
onto other protein domains.
This will be followed up
by some basic background
on natural zinc finger DNA interactions.
That's a lead-up into what has
become one of the real success stories
in protein design and engineering,
which is the designing
or engineering of specific DNA binders
or poly-zinc finger approaches.
I will then finish it up
with some recent progress
in the development
of specific protein-binding zinc fingers
to hopefully show you the potential
of this small versatile protein domain
as protein-binding targets