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1. Introduction
2. No nuclear-cytoplasmic Ca gradients
3. Transient nuclear-cytoplasmic Ca gradients
4. Confocal images of cytosolic Ca response
5. Ca gradient can be quite high
6. Mitochondrial staining with MitoTracker Green
7. Calcium waves propagation
8. The nuclear membrane is continuation of the ER
9. ER of pancreatic acinar cells
10. 2 known families of Ca releasing channels in ER
11. Nuclear envelope connected to ER
12. Isolated nuclei stained with EtBr
13. Ca uptake by the liver nuclear fraction
14. IP3-induced Ca release in liver nuclei
15. Further complications in Ca signaling hypothesis
16. Ca measurements inside the liver nucleus
17. The envelope is the only store of Ca in nucleus
18. Pancreatic nuclei as a useful model
19. Staining of nucleus with BODIPY FL ryanodine
20. Effect of IP3 and cADPR on Ca in nucleus
21. NAADP induced Ca release from the nuclei
22. NAADP, cADPR, IP3 and caffeine-induced release
23. NAADP and IP3 release Ca from the same store
24. 2-APB effect on Ca release
25. Ryanodine blocked NAADP/cADPR responses
26. Ca release in response to local uncaging of IP3
27. Ca measurements in the nucleoplasm
28. NAADP, cADP-ribose and IP3 induce Ca rise
29. Thapsigargin sensitive Ca store
30. Bafilomycin, brefeldin and nigericin pre-treatment
31. Effect of NAADP on IP3 and ryanodine receptors
32. Nuclear pore complexes are permeable to Ca (1)
33. Nuclear pore complexes are permeable to Ca (2)
34. Diffusion of Ca from perinuclear Ca puff sites
35. Ip3R and RyR in ER
36. Nuclear calcium signaling

Topics Covered

• Nuclear calcium signaling, an intriguing history of the last 10 years of research and promising recent developments with the emphasis on fluorescent confocal techniques
• Basic features and major consensus on principles
• Uncertainty about nuclear calcium independence
• New data on the elusive NAADP receptor

Links

Series:

• Calcium Signaling I

Categories:

• Biochemistry
• Cell Biology

Talk Citation

Gerasimenko, O. and Gerasimenko, J. (2007, October 1). Nuclear calcium signaling [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 15, 2025, from https://doi.org/10.69645/KKML4212.
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Publication History

• Published on October 1, 2007

Financial Disclosures

• Dr. Oleg Gerasimenko has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
• Dr. Julia Gerasimenko has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.