• Introduction to Calcium Signaling
• 72 min
  1. Introduction to cellular calcium signaling

  • Dr. Martin Bootman
• 20 min
  2. Monitoring Ca2+ concentration in living cells

  • Dr. Marisa Brini
• 30 min
  3. Cell boundary theorem

  • Prof. Eduardo Ríos
• Calcium Influx
• 19 min
  4. Arachidonic acid and store-independent Ca2+ entry

  • Dr. Luca Munaron
• 34 min
  5. Voltage-dependent calcium channels

  • Prof. Annette Dolphin
• 34 min
  6. Protein networks of voltage-gated Ca2+ channels – proteomic & functional analysis of local Ca2+ signalling

  • Prof. Bernd Fakler
• 37 min
  7. Intracellular Ca2+ signaling: calcium influx

  • Prof. Anant Parekh
• 60 min
  8. Molecular identification of the CRAC channel

  • Prof. Michael Cahalan
• 35 min
  9. TRP channels with diverse permeability profiles: regulation of blood pressure and fertility

  • Prof. Marc Freichel
• Calcium Release
• 53 min
  10. The InsP3 receptor calcium release channel

  • Prof. J. Kevin Foskett
• 31 min
  11. Molecular biology of ryanodine receptors: an overview

  • Dr. Christopher George
  • Prof. F. Anthony Lai
• 57 min
  12. cADPR and NAADP: messengers for calcium signalling

  • Prof. Antony Galione
• 43 min
  13. Ryanodine receptors and cardiac function

  • Prof. David Eisner
• Calcium Efflux and Sequestration
• 45 min
  14. Sodium-calcium exchange

  • Prof. John Reeves
• Organelle Calcium
• 42 min
  15. Regulation and role of mitochondrial Ca2+ homeostasis

  • Prof. Rosario Rizzuto
• 54 min
  16. Peroxisomes and Golgi apparatus as players in Ca2+ homeostasis

  • Dr. Paola Pizzo
  • Dr. Alex Costa
• 34 min
  17. Ca2+ dynamics between mitochondria and the endoplasmic reticulum

  • Dr. Wolfgang Graier
• 26 min
  18. Nuclear calcium signaling

  • Dr. Oleg Gerasimenko
  • Dr. Julia Gerasimenko
• Spatiotemporal Calcium Signals
• 54 min
  19. Regulation of intracellular calcium signaling, localized signals and oscillations

  • Prof. Barbara Ehrlich
• 43 min
  20. Modeling Ca2+ signals: understanding Ca2+ regulatory networks in cells: introduction and basic concepts

  • Dr. David Friel
• 41 min
  21. Modeling Ca2+ signals: understanding Ca2+ regulatory networks in cells - Ca2+ stores: endoplasmic reticulum, calcium oscillations

  • Dr. David Friel
• 49 min
  22. Modeling Ca2+ signals: understanding Ca2+ regulatory networks in cells - Ca2+ stores: mitochondria, models of spatially non-uniform Ca2+ signals, applications to analysis of experimental results

  • Dr. David Friel
• Calcium Effectors
• 34 min
  23. Neuronal calcium sensor proteins: calcium sensors and regulators of diverse cellular functions

  • Prof. Bob Burgoyne
• 41 min
  24. Calcium-regulated adenylyl cyclases and cyclic AMP compartmentalization

  • Prof. Dermot Cooper
• 32 min
  25. Calcium and transcription-coupling

  • Dr. Karen Lounsbury
• 50 min
  26. Cellular calcium (Ca2+) buffers

  • Prof. Dr. Beat Schwaller
• 24 min
  27. Extracellular calcium signaling

  • Dr. Aldebaran M. Hofer
• 50 min
  28. Ca2+, fertilization and egg activation

  • Prof. Karl Swann
• 24 min
  29. Calcium regulation of transcription in plants

  • Prof. Hillel Fromm
• 34 min
  30. Mechanisms regulating STIM expression and function in Ca2+ signaling

  • Dr. Jonathan Soboloff
• 46 min
  31. Dynamic signal encoding in the S. cerevisiae calcium response

  • Dr. Chiraj Dalal
• Calcium and Disease
• 43 min
  32. Polycystins, calcium signaling and pathogenesis of polycystic kidney disease

  • Prof. Laura del Senno
• 28 min
  33. Ca2+ alterations in familial Alzheimer's disease (FAD)

  • Dr. Paola Pizzo
• 38 min
  34. Pancreatitis and calcium signaling

  • Prof. Ole Petersen
• 47 min
  35. Mechanism-based therapies for heart failure and cardiac arrhythmias

  • Prof. Andrew Marks
• 46 min
  36. Genetic defects and calcium

  • Prof. Tullio Pozzan
• Archived Lectures *These may not cover the latest advances in the field
• 31 min
  37. Calcium, calmodulin and calcineurin

  • Prof. Stephen Bolsover
• 34 min
  38. Calcium flickers steer cell migration

  • Prof. Heping Cheng
• 28 min
  39. Automated Ca2+ imaging of chemosensory neurones in C.elegans

  • Dr. Nikos Chronis
• 46 min
  40. Ca2+ and the regulation of small GTPases

  • Prof. Peter Cullen
• 35 min
  41. Genetically encoded Ca2+ indicators: molecular scale measurements in mammals in vivo

  • Dr. Michael I. Kotlikoff
• 35 min
  42. Capacitative (store-operated) calcium entry

  • Dr. Jim Putney
• 47 min
  43. The molecular biology of the inositol trisphosphate receptor

  • Dr. Randen Patterson
• 50 min
  44. Coordinated Ca2+ release from intracellular Ca2+ stores

  • Prof. Ole Petersen
• 45 min
  45. The plasma membrane calcium pump: biochemistry, physiology and molecular pathology

  • Prof. Ernesto Carafoli
• 60 min
  46. The calcium saga: a matter of life and death

  • Prof. Pierluigi Nicotera
• 22 min
  47. Ca2+ efflux and Ca2+ signals

  • Dr. Anne Green
• 31 min
  48. Organellar calcium pumps of the golgi and ER: from yeast to human

  • Prof. Rajini Rao
• 58 min
  49. Spatial and temporal aspects of cellular calcium signals: 'elementary' signals and waves

  • Prof. Ian Parker
• 26 min
  50. Modeling Ca2+ signals

  • Dr. David Friel

Printable Handouts

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Navigable Slide Index

1. Introduction
2. Early view of Ca2+ signaling
3. Phylogenetic tree of eukaryotic Ca2+-ATPases
4. Current view of Ca2+ signaling
5. Why have a Ca2+ pump in the Golgi?
6. Studies in yeast
7. Deletion of pmr1 in yeast: phenotypes
8. Ca2+ signaling in yeast
9. Reverse genetics: from gene to protein to function
10. Engineering the host
11. Overexpression
12. Purification of (His)9-Pmr1
13. Ion-dependent ATPase activity
14. Calcium transport in Golgi fractions
15. Phenotype screening
16. Phenotype screen for Ca2+/Mn2+ transport activity
17. Growth screens of mutants
18. Ion selectivity mutants
19. Q783A transports Ca2+ but not Mn2+
20. Intragenic suppression
21. Compensation of Q783A phenotype
22. Molecular modeling: from structure to function
23. SERCA/ SPCA Ca2+/ Mn2+ binding sites
24. Alignment between PMR1 and SERCA1a
25. Ca2+ binding site
26. Conformation-sensitive packing of helices
27. Cross-sectional view of core helices
28. Space-filled view of core helices
29. Golgi pumps in mammals
30. SPCA1 characteristics
31. Cell-cell adhesion lost in Hailey-Hailey disease
32. hSPCA1 complements pmr1 null phenotypes
33. Molecular basis of disease: from yeast to human
34. hSPCA1 localizes to the Golgi
35. Ca2+ transport activity
36. hSPCA1 transports Ca2+ with a high affinity
37. All Ca2+ ATPases confer BAPTA tolerance
38. Only PMR1 and hSPCA1 confer Mn2+ tolerance
39. Ca2+-transport activity of SPCA pumps
40. HHD mutants in yeast lacking endogenous pumps
41. HHD missense mutations
42. Localization of mutations in the homology model
43. SPCA1 distribution in polarized cells
44. SPCA2 has a more restricted tissue distribution
45. Primary hippocampal neurons
46. Organellar Ca2+ pumps
47. Acknowledgements

Topics Covered

• Early and current views on Calcium signaling
• Phylogenetic tree of Calcium-ATPases
• Identification of a new family of Golgi/secretory pathway Calcium-ATPases (SPCA)
• Why have a Calcium pump in the Golgi?
• Yeast as a model to understand Golgi Calcium pumps
• Biochemical characterization of Calcium and Mn2+ transport
• Phenotype screening in yeast to identify residues important for ion transport and selectivity in Pmr1
• Structural and homology models of Calcium-ATPases
• Golgi pumps in mammals
• From structure to physiology
• Haploinsufficiency of ATP2C1 leads to Hailey Hailey disease
• Insights from yeast
• Distribution and localization of SPCA1 and SPCA2

Links

Series:

• Calcium Signaling I

Categories:

• Biochemistry
• Cell Biology

Talk Citation

Publication History

• Published on October 1, 2007
• Archived on February 25, 2021

Financial Disclosures

• Prof. Rajini Rao has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.