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1. Introduction
2. Outline
3. Background
4. Targets of free radical injury
5. Major source of free radicals in vivo is oxygen
6. Cellular sources of free radicals
7. Exogenous sources of free radicals
8. Antioxidants protect against oxidant stress
9. Lipid peroxidation is one form of oxidant stress
10. Lipid peroxidation and human disease
11. Diseases associated with lipid peroxidation
12. Methods to quantify lipid peroxidation
13. The ideal assay of lipid peroxidation
14. Assays of lipid peroxidation
15. Existing assays for quantifying lipid peroxidation
16. Fatty acid analysis
17. Fatty acids analysis in peroxidizing microsomes
18. Pros and cons of fatty acids analysis
19. Conjugated dienes
20. Conjugated dienes assay
21. Pros and cons of conjugated dienes assay
22. Lipid hydroperoxides
23. Plasma lipid hydroperoxides in SAH patients
24. Pros and cons of lipid hydroperoxides assay
25. Thiobarbituric acid-reactive substances /MDA
26. TBARS quantification in vitro versus in vivo
27. Alternative assays for TBARS quantification
28. TBARS levels in vivo
29. TBARS/MDA - summary
30. Alkanes
31. Pros and cons of alkanes assay
32. Alkanes formation in peroxidizing microsomes
33. F2-isoprostanes
34. Pathway of isoprostane formation
35. Analysis of F2-isoprostanes
36. Analysis of F2-isoprostanes in human plasma
37. Biological actions of F2-isoprostanes
38. Effect of 15-F2t-IsoP on renal function
39. F2-IsoPs formation in peroxidizing microsomes
40. CCl4-mediated oxidant injury
41. F2-IsoPs - markers of lipid peroxidation in vivo
42. F2-IsoPs as a measure of oxidant stress
43. Biomarkers of oxidative stress study (BOSS)
44. BOSS results
45. IsoPs levels and atherosclerosis
46. IsoP formation in humans - effect of smoking
47. Increases in BMI and IsoPs levels correlate
48. Effect of weight loss on IsoP formation
49. Vitamin E and cardiovascular disease trials
50. Reduction of IsoP formation by vitamin E
51. Vitamin C does not alter IsoP levels
52. Effect of fish oil supplementation on IsoP levels
53. Neurodegeneration and oxidant stress
54. F2-IsoP levels in CSF from AD patients
55. Study of CSF IsoP levels in AD patients
56. CSF IsoPs increase with AD progression
57. Antioxidants affect CSF IsoPs in AD patients
58. IsoPs and Alzheimer's disease: conclusions
59. Advantages of isoprostane quantification
60. Disadvantages of isoprostane quantification (1)
61. IsoPs increase selectively in AD patients' CNS
62. Disadvantages of isoprostane quantification (2)
63. Classes of IsoPs
64. Disadvantages of isoprostane quantification (3)
65. Immunoassay methods to quantify IsoPs
66. Conclusions (1)
67. Conclusions (2)

Topics Covered

• Oxidant stress, lipid peroxidation and related terms
• Basic mechanisms of lipid peroxidation and relevance to human physiology and pathophysiology
• Methods to quantify oxidative stress and lipid peroxidation
• F2-Isoprostanes (F2-IsoPs) as indices of oxidant stress in vivo
• Role as markers in human physiology and pathophysiology
• Effects of interventions on IsoP formation
• Advantages and disadvantages of isoprostane quantification

Links

Series:

• Free Radicals and the Oxygen Paradox

Categories:

• Biochemistry
• Cell Biology

Talk Citation

Morrow, J. (2007, November 1). Lipid peroxidation: from basic mechanisms to clinical relevance [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved November 23, 2024, from https://doi.org/10.69645/XUTC2653.
Export Citation (RIS)

Publication History

• Published on November 1, 2007
• Archived on May 31, 2018

Financial Disclosures

• Prof. Jason Morrow has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.