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Navigable Slide Index

1. Introduction
2. Bacterial pathways of protein secretion
3. Protein translocation across membranes
4. Major questions in protein translocation
5. "Signal hypothesis"
6. Essential requirements for protein translocation
7. Topogenic signal sequences
8. Factors for targeting, anti-folding and unfolding
9. Trigger factor - ribosome-associated chaperone
10. Bacterial SRP system
11. SecB is an export-specific chaperone
12. Identification of Sec factors by "forward genetics"
13. Biochemical approaches for Sec factors study
14. SecYEG homologs in higher organisms
15. SecY protein
16. SecY interacts with SecE and SecG
17. Structure of archaeal SecYE-beta translocon
18. Preprotein threads through the narrow channel
19. Gating devices of the translocon
20. Role of the lateral gate of the translocon
21. Two modes of translocon use
22. YidC-SecYEG cooperation
23. Higher order assemblies of SecYEG
24. SecYEG dimerization upon ribosome binding
25. SecYEG functions as a dimer
26. SecA is an ATPase
27. SecA protomer
28. What is the working form of SecA structure?
29. SecA insertion model
30. Two modes of SecY-SecA interactions
31. SecA ATPase as a power-controllable engine
32. SecM controls SecA at different levels
33. Translation of secM is subject to "arrest"
34. SecM contains an "arrest sequence"
35. SecM is required for expression of SecA
36. "Cis-chaperone" function of SecM
37. New concepts that SecM brought to us
38. Concluding remarks

Topics Covered

• Importance of protein translocation
• Identification of Sec factors
• Structure and function of translocon, an evolutionarily conserved membrane protein complex that serves as a polypeptide-conducting channel
• Structure and function of SecA, a translocation-driving ATPase
• Unique regulation of SecA by SecM, a protein that interacts with the ribosomal exit tunnel

Links

Series:

• Molecular Chaperones

Categories:

• Biochemistry
• Cell Biology

Talk Citation

Ito, K. (2007, October 1). The role of chaperones and Sec machinery in protein secretion [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved March 30, 2025, from https://doi.org/10.69645/IWAG2329.
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Publication History

• Published on October 1, 2007

Financial Disclosures

• Prof. Koreaki Ito has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.