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Printable Handouts
Navigable Slide Index
- Introduction
- Talk overview
- The present state of bacteriological monitoring
- Russell & Burch 1959
- History of commercial breeders
- Commercial breeders guidelines
- Contemporary prevalence of agents
- Research impact caused by Pasteurellaceae
- Pasteurella & Mychoplasma in murine pneumonia
- Back to the Charles Rivers survey
- The gut microbiota of rodents
- 1 mouse + 1000 different germs
- Cultivation of bacteria present in the gut
- Microbiomics
- PCR based methods
- Impact of the gut microbiota on rodent models
- Isolators in room facility
- Impact of germ free perinatal period
- Gut microbiota composition and disease associations
- Obesity associated gut microbiome
- Gut microbiota and glucose tolerance
- Ampicilin-improve glucose tolerance
- Mechanisms behind gut microbiota impact
- Acquired and innate immunity
- Gut microbiota, immune system: early encounter
- MAMPs & TLRs
- Early life vancomycin treatment in NOD mice
- Early life priming of the regulatory immunity
- Mechanisms behind Inflammatory Bowel Disease
- Taconic mice contain pro-inflammatory bacteria
- Diabetes in SFB-negative and SFB-positive NOD mice
- The gut microbiota as therapeutic target
- Monosaccharide channel hypothesis
- GPR 41/43 hypothesis
- Bacterial enzyme hypothesis
- Gut-brain exis
- The gut microbiota as a cause of variation
- The principal component analysis (PCA)
- Mouse and human microbiome comparison
- Microbiota composition & research parameters
- Variation in the gut microbiota
- Group sizes and power in animal experiments
- Impact of halving variation and doubling effect
- Gut microbiota in leptin-deficient obese mice
- Gut microbiota and local inflammation
- How to handle gut microbiota impact
- Be aware of environmental & dietary fluctuations
- Gut microbiota composition and stress
- Dietary impact on gut microbiota and behaviour
- Oligosaccharids and gut microbiota
- Dietary starch regulates inflammatory responses
- Impacts of gluten-free diet om NOD mice
- Impacts of maternal gluten-free diet
- Family relationship of female breeders
- Selective inbreeding and gut microbiota similarity
- Mode of delivery shapes gut colonization pattern
- Incorporate microbiota in experimental design
- Environmental effects on microbiota composition
- Controlling variation
- Rodents with a tailored-made gut microbiota
- Start out with inoculated germ-free mice
- The vision for a future laboratory rodent
- Early gut colonization & future immune responses
- Transfer of microbiota from lean and obese mice
- Gut Immune maturation depends on colonization
- The future: robot-maintained facilities
- Do you think this is science fiction?
- Conclusions
- Conclusion The gut microbiota
- Acknowledgements
Topics Covered
- Bacteriological monitoring in lab rodents
- Impact of the gut microbiota on rodent models
- The interaction between the host and gut microbiota
- The gut microbiota as a cause of variation in rodent models
- Handle the impact: environmental & dietary fluctuations
- Handle the impact: rodents with tailored-made gut microbiota
Links
Series:
Categories:
Talk Citation
Hansen, A.K. (2015, February 11). Improving and humanizing animal models by microbiomic techniques [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved December 6, 2024, from https://doi.org/10.69645/WWJE1342.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Axel Kornerup Hansen has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
Other Talks in the Series: Animal Models in Biomedical Research
Transcript
Please wait while the transcript is being prepared...
0:00
Hello, my name is
Axel Kornerup Hansen.
I'm a professor and the
board chairman of science
at the University of Copenhagen.
In this lecture, I will
introduce you to the impact
that the microbiome
has on animal models,
and how we can improve
your work with these models
by considering these aspects.
In principle, this could be
about models in various species.
But I will focus on rodents, because
they outnumber other model species,
because there are
those who are primarily
used for their low variation, and
high potential for standardisation,
and also because this is
what I'm actually working
with in my own research group.
0:36
In this lecture, I will
start out by talking
about how bacteriological monitoring
is done in laboratory rodents
today.
And after that, I will introduce
to the fascinating world
of the gut microbiota.
I will talk about the impact
that this microbiota has
in rodent models, and give
a short and very incomplete
impression on how this interaction
between the host and the microbiota
may take place.
And introduce you to how
much variation actually
causes in your rodent models,
and I will end up presenting
some plausible ways
to deal with this impact.
1:13
Bacteria are a source of variation
in laboratory animal work.
This is not a new issue,
and the systematic handling
of the variation caused
by bacteria has developed
from the beginning of the 20th
century, through the millennium,
and up till today.
However, there's still
much which can be done.
So let me start out by shedding
a critical light on the states
at which we are today in
laboratory animal breeders,
be it facilities,
or be it scientists.