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1. Introduction
2. Origins and diversity of the basic animal body plan
3. Ernst Haeckel, Anthropogenie
4. Ernst Haeckel “Tree of life”
5. Most vertebrate embryos look the same
6. How does diversity follow from similarity?
7. Hox homeotic genes
8. A common origin
9. Vertebrate Hox genes co-linearity and expression
10. Combinatorial “Hox code”
11. Conservation of Hox genes in vertebrates
12. Common genes in different organisms
13. The phylotypic stage
14. Hox genes and axial identity in development
15. The vertebrate hindbrain and Hox genes
16. Patterning brain and facial development
17. Segmental organization of the hindbrain
18. Conservation of hindbrain segmentation
19. Organization of the hindbrain
20. Alternating segmental properties and segmentation
21. Transcriptional profiling individual rhombomeres
22. Segmental patterns of gene expression
23. Monitoring nested Hox expression
24. Segmental organization of the hindbrain
25. Hox genes regulate segmental identity
26. Hox genes regulate multiple steps of segmentation
27. The nervous system and craniofacial development
28. Formation and fate of neural crest cells
29. Hindbrain segmentation and neural crest migration
30. Neural crest cells migration and differentioation
31. Segmental organization of the hindbrain
32. How are AP patterns of Hox expression set up?
33. Hox genes and axial identity specification
34. Analysis of cis-regulation of HoxB genes
35. Segmental hindbrain HoxB expression regulation
36. Modular cis-regulatory control of Hoxa2
37. Hindbrain cis-regulatory modules of Hox genes
38. Vertebrate hindbrain segmentation GRN
39. Regulation of vertebrate hindbrain organisation
40. Common role for auto/cross-regulation
41. Previously characterized Hox/Pbx binding sites
42. The rhombomere 4 regulatory network
43. DV patterns of Hox expression
44. Segmental and neuronal functions of Hox genes
45. Complex cis-regultory landscape
46. Hox genes as morphogenic information integrators
47. Opposing signaling centers drive axial extension
48. Regulators of Hox gene expression
49. Hox expression regulation and retinoids
50. Where are the RAREs and how do they function?
51. HoxB multiplex reporter BAC
52. RAREs are necessary for expansion of 5’ Hoxb
53. Summary: Hox gene regulation by multiple RAREs
54. Conserved and unique RAREs in Hox clusters
55. Is DE-RARE is responsible for anterior expansion?
56. DE-RARE can anteriorize 5’ Hoxa gene expression
57. Hox genes, retinoids and heart development
58. Retinoids and Hox genes in heart development
59. Deletion of the HoxA and HoxB complexes
60. Where are the heart regulatory regions
61. Identification of novel HoxB control regions
62. Novel RAREs in heart and endoderm enhancers
63. Identification of novel HoxB control regions
64. Cooperative/shared/selective/long-range enhancers
65. Building the gene regulatory network
66. Acknowledgements
67. Stowers Institute

Topics Covered

• Origins and diversity of the basic animal body plan
• Ernst Haeckel: Anthropogenie, “Tree of life”
• Common mechanisms for vertebrate body plan
• How does diversity follow from similarity?
• Hox homeotic genes are clustered and control head to tail patterning
• Vertebrate Hox genes display co-linear and segmental expression
• “Hox code”
• Conservation of Hox genes in vertebrates
• The phylotypic stage
• The vertebrate hindbrain, head development & Hox genes: A story in segments
• The nervous system plays an important role in regulating craniofacial development through formation of neural crest cells
• Roles for hindbrain segmentation & neural crest migration in craniofacial patterning
• How are the AP patterns of Hox expression set up?
• Analysis of cis-regulation of HoxB genes
• Modular cis-regulatory control of Hoxa2
• Vertebrate hindbrain segmentation GRN
• Regulation of vertebrate hindbrain organisation
• Previously characterized consensus Hox/Pbx binding sites
• The rhombomere 4 regulatory network
• DV patterns of Hox expression
• Segmental and neuronal functions of Hox genes
• Complex cis-regultory landscape controls Hox gene expression in the specification of axial identity
• Hox genes as integrators of morphogenic information
• Opposing signaling centers drive axial extension: Wnt and Fgf signaling oppose RA signaling
• Regulators of Hox gene expression
• What mechanisms do retinoids use to regulate Hox expression?
• Where are the RAREs and how do they function in the Hox clusters?
• HoxB multiplex reporter BAC reproduces endogenous gene expression pattern
• RAREs are necessary for anterior expansion of 5’ Hoxb expression in the neural tube
• Conserved and unique RAREs in Hox clusters
• Is DE-RARE responsible for the anterior expansion?
• DE-RARE can anteriorize 5’ Hoxa gene expression
• Hox genes, retinoids and heart development
• Where are the heart regulatory regions
• Identification of novel HoxB control regions
• Novel RAREs in heart & endoderm enhancers
• Cooperative, shared, selective and long-range enhancers
• Building the gene regulatory network which governs segmental, neuronal and patterning functions of Hox genes

Links

Series:

• Epigenetics, Chromatin, Transcription and Cancer

Categories:

• Biochemistry
• Cell Biology
• Genetics & Epigenetics

Talk Citation

Publication History

• Published on September 3, 2014

Financial Disclosures

• Prof. Robb Krumlauf has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.