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Printable Handouts
Navigable Slide Index
- Introduction
- Overview
- Notch receptor discovered in Drosophila
- Role of Notch in developing wing margin
- Role of cut in the development of adult wing margin
- cut and Ubx are sensitive to Nipped-B dosage
- Cohesin and the Nipped-B loading factor
- Sister chromatid cohesion cycle
- Nipped-B is required for sister chromatid cohesion
- Cornelia de Lange syndrome (CdLS)
- Investigating hypothesis and approach
- Genome-wide chromatin immunoprecipitation
- Nipped-B co-localizes with cohesin genome-wide
- Nipped-B and cohesin overlap RNA Pol-II (1)
- Histone modifying complexes
- Rad21 is a trithorax group gene (verthandi)
- Bithorax complex gene map
- Nipped-B & cohesin at transcription start sites
- Effects of Nipped-B & cohesin on gene expression
- Nipped-B and cohesin regulate the same genes (1)
- Cohesin: positive and negative direct regulation
- Cohesin and control of development & translation
- Nipped-B and cohesin regulate the same genes (2)
- Nipped-B and cohesin overlap RNA Pol-II (2)
- Thomas Hunt Morgan
- Invected-engrailed homeobox gene complex
- Enhancer of split anti-neural gene complex
- Genes in rare regions of cohesin-H3K27me3
- E(spl)-C partially repressed by Polycomb
- Cohesin regulates genes that it binds
- Question & approach to solution
- Cohesin-binding genes (1)
- Cohesin-binding genes (2)
- GTGTG is enriched only in the plus strand
- GAGAG is binding site for GAGA factor (GAF)
- Nipped-B binding predicts GAF binding
- Genes with paused Pol-II produce short RNA
- Cohesin-binding genes (3)
- Elongating polymerase
- Nipped-B and cohesin in polymerase pausing (1)
- Nipped-B and cohesin in polymerase pausing (2)
- RNA polymerase likely passes through cohesin
- How does cohesin repress transcription?
- Genes strongly repressed by cohesin (1)
- Genes strongly repressed by cohesin (2)
- Genes strongly repressed by cohesin (3)
- Genes strongly repressed by cohesin (4)
- Cohesin doesn’t block binding of Pol-II
- Could cohesin magnify pausing factor activity?
- Pausing factor depletion effects
- NELF & Spt5 in pausing and elongation
- Cohesin role in pausing and elongation
- Cohesin can inhibit and facilitate elongation
- Spt5 knockdown does not reduce cohesin binding
- GAF and GT repeats recruit NELF and cohesin
- Summary (1)
- Summary (2)
- Questions that remain to be answered
- Acknowledgements
- Lab members
- Research supporters
Topics Covered
- Cohesin Regulates Gene Expression and Development in Drosophila and Humans
- Cohesin Binds a Subset of Active Genes at Transcription Start Sites
- Cohesin Directly Regulates Many Genes Important for Growth and Development
- Cohesin Cooperates with Polycomb Epigenetic Silencing Complexes to Restrain Gene Expression
- Cohesin Selectively Binds Genes with Paused RNA Polymerase and Regulates Transition to Transcriptional Elongation
- Questions Regarding the Roles of Cohesin in Gene Regulation and Development and Relevance to Human Disease
Links
Series:
Categories:
Talk Citation
Dorsett, D. (2014, February 4). Genomic insights into gene regulation by cohesin [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved December 3, 2024, from https://doi.org/10.69645/ZRPQ4920.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Dale Dorsett has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
A selection of talks on Biochemistry
Transcript
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0:00
My name is Dale Dorsett from
the Department of Biochemistry
and Molecular Biology at the
Saint Louis University School
of Medicine, and I'm going
to talk about gene regulation
by cohesin in Drosophila,
which has provided
insights into certain
human conditions.
0:18
In my talk, I'm going to
cover four major points.
The first is, is that sister
chromatids cohesion proteins
regulate many genes that
are important for growth
and development?
The second point is going to be
about a specific mechanism by which
cohesin controls gene expression,
and that said, cohesin collaborates
with Polycomb silencing
proteins to restrain,
but not actually silence genes.
We're also going to talk about
how cohesin binds genes that have
paused RNA polymerase, and how it
controls the ability of that paused
polymerase to become
elongating polymerase.
And finally, I'm going to summarize
how gene regulation by cohesin
can explain diverse
developmental deficits that occur
in a human genetic syndrome,
Cornelia de Lange syndrome.
1:08
For my laboratory, the
entry point into this work
was an attempt to explain the
wing-nicking phenotype displayed
by the original Notch receptor
mutation discovered many years ago
by Thomas Hunt Morgan and
Calvin Bridges, in Drosophila.
1:26
Work by many laboratories
over several years
had led to the realization that the wing-nicking phenotype in Notch mutants
probably derives from underexpression of the cut gene
during the third instar
larval development,
along the presumptive
future wing margin.
So activation of the Notch receptor
in dorsal and ventral cells
activates cut in a thin
stripe of cells that
will eventually form
the adult wing margin.