Intestinal stem cell-mediated repair in Drosophila 2

Published on January 5, 2014 Reviewed on September 25, 2017   30 min

Other Talks in the Category: Cell Biology

0:04
The last set of slides of TSC2 function illustrates an intrinsic regulatory mechanism for stem cells, in this case, for growth and division. The other intrinsic mechanisms that regulate asymmetry, renewal, or apoptosis are being pursued by many different laboratories. In the next set of slides, we're going to discuss how short-range interaction with surrounding cells might regulate stem cell division.
0:33
Some insights of how stem cells interact with immediate environment, usually called a niche, came from our earlier study using different tissue damaging agents. In the case of dextran sulfate sodium, or DSS, it is a widely used chemical to induce inflammatory bowel diseases in mammalian experimental systems, like a mouse or rat. Dextran sulfate sodium is a synthetic polysaccharide that has multiple positive charges. Feeding DSS to animals can cause injury that is compounded by the presence of commensal bacteria, inflammation, cytokine production, and lymphocyte attractions that cause serious tissue damage after inflammation. It is a widely used model to study human ulcerative colitis. Feeding of DSS to fruit flies, as shown in the lower panel, can kill flies within days, depending on dosage that we used. At early time points of day 1 and 2 and 3, after feeding DSS, we could already detect very highly increased mitotic activity inside the drosophila midgut.
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Intestinal stem cell-mediated repair in Drosophila 2

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