mRNA splicing and post-transcriptional modifications

In this talk, we turn our attention to RNA splicing and post transcriptional modifications, framing our discussion around the role of messenger RNA in translating genetic information from DNA to protein, highlighting the complex processing steps required to generate functional RNA. We will discuss the importance of RNA splicing, including how introns are removed and exons joined by the splicosome and examine the significance of alternative splicing in generating proteomic diversity. Key modifications such as five prime capping and three prime polyadenylation will be outlined for their roles in MRNA stability and function. Finally, we will explore how proper RNA processing is essential for health and how its dysregulation can lead to disease. Messenger RNA or RNA serves as the link between genetic information in DNA and protein synthesis. The path from gene to functional RNA is complex, involving several processing steps that impact gene expression and protein diversity. In eukaryotic cells, the initial RNA transcript called pre messenger RNA, undergoes modifications like intron removal, exon joining, and the addition of protective structures, all of which ensure accuracy, stability, and versatility in gene expression. One of the most vital steps in RNA processing is pre messenger RNA splicing, which excises non coding introns and lgates coding exons to create a continuous open reading frame. Introns are often longer than exons and are