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1. Introduction
2. Finding key conserved "disease" genes
3. Drosophila: good model for human "disease" genes
4. VDRC genome wide RNAi in Drosophila
5. Using the RNAi library
6. Pain behavior in fruit fly
7. Finding conserved regulators of nociception
8. High temperature is noxious to Drosophila
9. Assay for nociception in adult flies
10. Drosophila rapidly avoid high temperature
11. Painless mutant flies & noxious temperatures
12. Nociception screening system in adult fly
13. Genome wide nociception screen in adult fly
14. Screening data (pathway enrichment)
15. Human GWAS for chronic pain after back surgery
16. Human GWAS vs. calcium signaling
17. Straightjacket (stj) is a novel fly "pain" gene
18. stj is found in brain & peripheral sensory neurons
19. alpha-2-delta-3 (stj): Drosophila larvae "pain" gene
20. alpha-2-delta-3 mutant larvae
21. Generation of alpha-2-delta-3 KO mice
22. KO mice show delayed nociception responses
23. alpha-2-delta-3 KO mice basic phenotype
24. The pain circuit
25. Problem - no difference in a tail flick assay
26. alpha-2-delta-3 may act in the brain
27. KO mice: possible altered pain processing in brain
28. fMRI setup
29. alpha-2-delta-3: thalamus to SSC transmission
30. Problem - there is overall normal total brain activity
31. Loss of alpha-2-delta-3
32. alpha-2-delta-3 (stj): an acute & chronic pain gene
33. Conclusions
34. Acknowledgements

Topics Covered

• Finding key conserved pain genes in drosophila as a model for human genes
• Finding conserved regulators of nociception
• Assay for nociception in adult flies
• Painless mutant flies & noxious temperatures
• Genome wide nociception screen in adult fly
• Human GWAS for chronic pain gene
• Alpha-2-delta-3 (Straightjacket = stj) as a novel acute & chronic pain gene and the effect on its manipulation in mice and flies

Links

Series:

• Calcium Signaling II

Categories:

• Biochemistry
• Cell Biology

Therapeutic Areas:

• Neurology

Talk Citation

Publication History

• Published on October 31, 2012

Financial Disclosures

• Dr. Greg Neely has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.