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1. Introduction
2. Transcription factors in macrophage differentiation
3. Differentiated cells
4. Definition by function
5. Definition by phenotype
6. Definition by ontogeny
7. Hematopoietic lineage
8. Macrophage lineage
9. Macrophage transcription factors
10. M-CSF cytokine sensitivity
11. Transcription factor control
12. Gain of function vs. loss of function mutations
13. C/EBPalpha KO
14. PU.1 KO (1)
15. PU.1 KO (2)
16. Early lineage commitment
17. Commitment
18. Transcription factor-cytokine interaction
19. Ectopic expression (1)
20. Ectopic expression (2)
21. Ectopic expression (3)
22. Summary I
23. Levels
24. Cross inhibition
25. Balance (1)
26. Balance (2)
27. Balance (3)
28. Successive competition
29. Combinatorial control
30. Integrated inhibition and cooperation
31. Changing complexes
32. Summary II
33. Enhancer definition
34. Cell type specific enhancers
35. ChIP-Seq enhancer definition
36. Comparing enhancers from different cell types
37. Cell type specific enhancer signature (1)
38. Cell type specific enhancer signature (2)
39. Global combinatorial enhancers
40. Polarization
41. Summary III
42. Control of cellular proliferation during differentiation
43. Recalling hematopoietic differentiation
44. Maf transcription factors
45. Maf transcription factors and cell differentiation
46. Maf overexpression
47. MafB overexpression
48. Maf/Myb in macrophage differentiation
49. Summary IV
50. Differentiation and cell cycle arrest are linked
51. MafB-/- macrophage differentiation
52. MafB,c-Maf parallel expression
53. MafB/c-Maf DKO macrophage differentiation
54. Differentiation and cell cycle arrest are linked
55. Differentiation & cell cycle arrest dissociation (1)
56. Differentiation & cell cycle arrest dissociation (2)
57. Mature adult macrophages
58. Unlimited self renewal
59. What is self renewal?
60. Maintained macrophage identity
61. Functional tissue integration
62. Non tumorigenic cells
63. c-Myc and KLF-4 dependence
64. Summary V
65. Thank you
66. References (1)
67. References (2)
68. References (3)
69. References (4)
70. References (5)

Topics Covered

• Control of cellular identity and cell numbers
• Gain and loss of function approaches
• Transcription factor/cytokine interplay
• Mechanism: transcription factor levels, balance and combinatorial control
• Enhancer definition of macrophage identity
• Differentiation and cell cycle control

Links

Series:

• Macrophage Heterogeneity and Function

Categories:

• Biochemistry
• Cell Biology
• Immunology

Therapeutic Areas:

• Immunology & Inflammation

Talk Citation

Sieweke, M. (2012, June 14). Transcription factors in macrophage differentiation [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 15, 2025, from https://doi.org/10.69645/USGV8552.
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Publication History

• Published on June 14, 2012

Financial Disclosures

• Dr. Michael Sieweke has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.