• Overview of Chaperone Networks
• 41 min
  1. Mapping the molecular chaperone interaction network in yeast

  • Prof. Walid A. Houry
• 30 min
  2. The interaction network of the GroEL chaperonin

  • Prof. Dr. F. Ulrich Hartl
• 61 min
  3. Human heat shock protein families

  • Prof. Herman Kampinga
• 42 min
  4. Extracellular proteostasis: an emerging field

  • Prof. Mark Wilson
• 36 min
  5. Protein homeostasis and the chaperone interaction network in Arabidopsis thaliana

  • Prof. Rongmin Zhao
• Proteasome Networks
• 64 min
  6. Biogenesis of the eukaryotic proteasome

  • Prof. Mark Hochstrasser
• Understanding Intrinsically Disordered Proteins in Protein Homeostasis
• 36 min
  7. Intrinsically unstructured proteins: regulation and disease

  • Dr. M. Madan Babu
• 43 min
  8. The roles of intrinsic disorder in protein interaction networks

  • Prof. Vladimir N. Uversky
• Gene Regulatory Networks and their Role in Protein Homeostasis
• 38 min
  9. Network motifs: basic building blocks of biological networks

  • Prof. Uri Alon
• 41 min
  10. Structure, evolution and dynamics of gene regulatory networks

  • Dr. M. Madan Babu
• 33 min
  11. Computational methods in analysis of gene regulation and protein interactions

  • Prof. Ron Shamir
• 51 min
  12. Protein-protein interaction networks

  • Prof. Peter Csermely
• Protein Homeostasis in the Endoplasmic Reticulum
• 56 min
  13. Role of calnexin and calreticulin in protein homeostasis within the endoplasmic reticulum

  • Prof. David B. Williams
• 30 min
  14. The unfolded protein response

  • Prof. Kazutoshi Mori
• 38 min
  15. Role of ER stress in cystic fibrosis airway inflammation

  • Dr. Carla Maria Pedrosa Ribeiro
• 57 min
  16. The recognition of misfolded glycoproteins in the endoplasmic reticulum

  • Dr. David Y. Thomas
• 49 min
  17. Chaperone systems of the endoplasmic reticulum

  • Prof. Linda M. Hendershot
• 74 min
  18. The ERAD network

  • Prof. Daniel Hebert
• Protein Homeostasis in the Mitochondria
• 22 min
  19. Protein homeostasis in mitochondria: AAA+ chaperones & proteases

  • Dr. David A. Dougan
• 46 min
  20. Protein homeostasis in mitochondria: chaperones and proteases of the mitochondrial matrix

  • Prof. Wolfgang Voos
• 26 min
  21. Mitochondrial chaperonin Hsp60: locations, functions and pathology

  • Prof. Francesco Cappello
  • Prof. Alberto J. L. Macario
• Protein Homeostasis in the Nucleus
• 23 min
  22. Nuclear protein quality control degradation

  • Dr. Richard G. Gardner
• Protein Homeostasis in Aging Disease
• 27 min
  23. Protein homeostasis during ageing: C. elegans as a model organism

  • Prof. Nektarios Tavernarakis
• Protein Homeostasis in Neurodegeneration
• 41 min
  24. Endoplasmic reticulum stress in neurodegenerative diseases

  • Prof. Claudio Soto
• 48 min
  25. Roles for Hsp40 molecular chaperones in protein misfolding disease

  • Prof. Douglas M. Cyr
• 38 min
  26. Protein folding in vivo

  • Prof. James Bardwell
• 39 min
  27. Protein degradation and defense against neurodegenerative disease 1

  • Prof. Alfred Goldberg
• 42 min
  28. Protein degradation and defense against neurodegenerative disease 2

  • Prof. Alfred Goldberg

Printable Handouts

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Navigable Slide Index

1. Introduction
2. Principles of mitochondrial protein homeostasis
3. Enzymatic mediators of protein homeostasis
4. Protein quality control in the matrix compartment
5. Question and approaches
6. In organello degradation assay
7. The mitochondrial protease Pim1
8. Degradation rates of imported reporter proteins
9. Folding state effects mitochondrial proteolysis
10. Biochemical properties of the protease Pim1
11. Substrate selectivity of the protease Pim1
12. 2D gel of mitochondrial proteins
13. Proteomic analysis of the protein turnover
14. Quantitative analysis of protein spot pattern
15. Proteolysis in organello
16. Pim1 substrates
17. Degradation in Fe/S cluster assembly mutants
18. Protein dynamics under oxidative stress
19. Protein degradation in ROS stressed mitochondria
20. Quantitative changes in mitochondrial proteome
21. Proteins with Fe/S cluster cofactors
22. In organello degradation of imported Ilv3
23. ROS-protective enzymes
24. Prx1 (peroxiredoxin)
25. Effect of oxidative stress on protein aggregation
26. Mitochondrial proteins affected by aggregation
27. Enzyme activities of affected proteins
28. Energy dependence of protein aggregation
29. Mitochondrial chaperone system protective effects
30. Pim1/LON protease protects against aggregation
31. Chaperones of the Hsp100/ClpB family
32. Reversal of protein aggregation
33. Resistance to oxidative stress in vivo
34. The mitochondrial protein quality control reaction
35. Acknowledgements

Topics Covered

• Protein homeostasis in mitochondria: the role of chaperones and proteases
• Principles of mitochondrial protein homeostasis
• Protein quality control in the matrix compartment
• Substrate selectivity of the mitochondrial protease Pim1
• Proteomic analysis of mitochondrial protein turnover
• Proteome alterations under oxidative stress
• Identification of aggregation-prone polypeptides
• Protective effects of chaperones
• Cooperation of Hsp78 and Pim1 in removal of aggregated polypeptides

Links

Series:

• Protein Homeostasis

Categories:

• Biochemistry
• Cell Biology

Talk Citation

Publication History

• Published on February 2, 2012

Financial Disclosures

• Prof. Wolfgang Voos has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.