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1. Introduction
2. Threats from pathogens
3. Strategies to treat infections
4. Antibiotic resistance
5. E. coli MarR
6. Bacterial multidrug resistance regulators
7. MarR family transcriptional factors in S. aureus
8. Susceptibility of S. aureus mutants to NOR
9. Resistance to quinolone for S. aureus strains
10. Virulence test using the murine abscess model
11. Crystal structure of MgrA
12. Molecular mechanism for MgrA-based regulation
13. A unique Cys residue
14. Oxidation sensing?
15. Cysteine sulfenic acid formation in MgrA
16. Gel-shift assay to study MgrA-DNA interaction
17. Bio-relevance: plate sensitivity assays
18. Further in vivo results
19. MgrA is a redox switch in S. aureus
20. SarZ, an MgrA homologue in S. aureus
21. SarZ is also an oxidation sensor
22. SarZ plays a complementary role next to mgrA
23. Crystal structure of the reduced SarZ
24. Crystal structure of oxidized SarZ with Cys13-SOH
25. SarZ and mgrA are major ROS in S.aureus
26. How S. aureus senses host immune defense
27. High virulence/low virulence hypothesis
28. Virulence suppression strategies
29. PVS works like hydrogen peroxide
30. An epoxide as a mild and selective alkylator
31. Phenyl epoxide sulfonate (PES) activates MgrA
32. The in vivo effect of the epoxide
33. Developing small molecule modulators
34. FA-based HTS for non-covalent modulators
35. NSRB at Harvard
36. MgrA homologues in other pathogens
37. Two MgrA homologues in P. aeruginosa
38. OspR in P. aeruginosa
39. Cys24 in OspR is redox active
40. OspR plays a role in virulence
41. P. aeruginosa MexR
42. MexR involved in regulating resistance: hypothesis
43. MexR oxidation leads to its dissociation from DNA
44. Intermonomer disulfide bond formation in MexR
45. Computational analysis of oxidized MexR structure
46. Redox potential measurement of MexR
47. Experiments probing in vivo response
48. MexR senses peroxide stress
49. Crystal structure of the oxidized MexR
50. Conclusion
51. Acknowledgements

Topics Covered

• Virulence regulation
• oxidative stress from immune response can dramatically affect the virulence in certain human pathogens-antibiotic resistance-oxidative stress induced by antibiotic treatment can affect resistance-Staphyloccocus aureus-Pseudomonas aeruginosa

Links

Categories:

• Cell Biology
• Microbiology
• Pharmaceutical Sciences

Therapeutic Areas:

• Infectious Diseases

Talk Citation

He, C. (2011, March 15). Virulence and antibiotic resistance regulation in pathogens [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved November 23, 2024, from https://doi.org/10.69645/HSDF1712.
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Publication History

• Published on March 15, 2011

Financial Disclosures

• Prof. Chuan He has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.