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1. Introduction
2. Survival for the major cancer killers in the US
3. Epigenetics versus genetics
4. Methylation of cytosine
5. Structure of the known DNA methyltransferases
6. Histone modifications in promoters during cancer
7. Cytosine methylation and chromatin remodeling
8. Gene specific promoter hypermethylation
9. Biological fluids and cancer detection
10. Methylation specific PCR
11. Methylation specific PCR assay
12. Two step methylation specific PCR
13. Real time assay for detecting methylation
14. Challenges for methylation-based assays
15. Lung cancer
16. p16 methylation during carcinoma development
17. Gene promoter methylation as a biomarker
18. Previous studies continued
19. Strategy for early detection of lung cancer
20. Goal (1)
21. Colorado cohort study of smokers with COPD
22. Colorado nested, case control sputum study
23. Candidate methylation biomarkers evaluated
24. Prevalence for gene methylation in sputum (1)
25. Sputum evaluation and cancer diagnosis
26. Key question
27. Prevalence for gene methylation in sputum (2)
28. Gene panel methylation in cancer detection (1)
29. Conclusions and future strategy
30. Gene promoter methylation in plasma
31. Goal (2)
32. Summary of selected variables
33. Prevalence for gene methylation in plasma (1)
34. Prevalence for gene methylation in plasma (2)
35. Prevalence for gene methylation in sputum
36. Gene panel methylation in cancer detection (2)
37. Conclusions (1)
38. Gene promoter methylation: utility for diagnosis
39. Methylation of p16 and H-cadherin
40. Emerging applications
41. Colon cancer detection in stool
42. Bladder cancer detection in urine
43. Prostate cancer diagnosis and staging
44. Prostate cancer detection in urine
45. Conclusions (2)
46. Thank you

Topics Covered

• How genes are silenced epigenetically via cytosine methylation and chromatin remodeling
• Assays for detecting gene promoter methylation in biological fluids
• Challenges for developing methylation-based assays
• Lung cancer case-control study of gene promoter methylation in sputum
• Predicting risk for lung cancer through gene methylation detection in plasma
• Gene promoter methylation as a diagnostic tool for lung, colon, prostate, and bladder cancer

Links

Series:

• DNA Methylation

Categories:

• Cancer
• Cell Biology
• Clinical Practice
• Genetics & Epigenetics

Therapeutic Areas:

• Oncology

Talk Citation

Belinsky, S. (2010, July 1). DNA methylation as a tumor marker: implications for diagnosis, early detection and risk assessment [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 18, 2024, from https://hstalks.com/bs/1747/.
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Publication History

• Published on July 1, 2010

Financial Disclosures

• Prof. Steve Belinsky has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.