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1. Introduction
2. Mitochondrial heterogeneity in cells
3. Mitochondrial function
4. Multiple levels of heterogeneity
5. Sub-cellular heterogeneity
6. Membrane potential - JC1
7. Membrane potential - TMRE
8. Imaging the permeability transition pore
9. Subcellular heterogeneity in PTP
10. Calcium: a ubiquitous messenger
11. Mitochondrial calcium handling
12. Measuring mitochondrial calcium in live cells
13. Agonist evoked calcium signals
14. Graded mitochondrial response
15. Calcium uptake by mitochondria
16. Functionality summary
17. Morphology confusion
18. Fission and fusion
19. How many mitochondria?
20. Luminal continuity
21. Electrical continuity
22. Mitochondria continuity assays
23. 3D reconstructions of mitochondria in live cells
24. Fluorescence recovery after photobleaching
25. DsRed1 mobility
26. DsRed and TMRE
27. Irradiation induced depolarisation
28. Hepatocyte electrical discontinuity
29. HeLa cell discontinuity
30. HeLa cell map
31. Pancreatic acinar and HUVEC maps
32. Cardiac myocyte electrical discontiuity
33. Oxidative damage
34. Electrical discontinuity summary
35. Atypical networks - electrical discontinuity
36. Atypical networks - FRAP
37. Atypical networks summary
38. Final summary
39. Acknowledgement
40. Selected references

Topics Covered

• Levels of mitochondrial heterogeneity
• Subcellular heterogeneity
• Membrane potential
• Permeability transition
• Calcium uptake
• Morphology: network or discrete?
• Live cell mitochondrial 3D reconstruction
• Fluorescence Recovery After Photobleaching (FRAP)
• Electrical continuity
• Atypical networks

Links

Series:

• Mitochondrial Biogenesis

Categories:

• Cell Biology

Talk Citation

Publication History

• Published on October 1, 2007
• Archived on May 31, 2018

Financial Disclosures

• Dr. Tony Collins has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.