• Cellular Mechanisms of Mitochondrial Biogenesis in Health
• 23 min
  1. Nuclear control of electron transport chain gene expression

  • Dr. Glenn C. Rowe
• 48 min
  2. Regulation of mitochondrial biogenesis by transcriptional coactivators and estrogen-related receptor alpha

  • Dr. Anastasia Kralli
• 17 min
  3. Protein import into mitochondria

  • Dr. Trevor Lithgow
• 39 min
  4. Mitochondrial phospholipid synthesis and incorporation

  • Prof. Grant Hatch
• 23 min
  5. Mitochondrial DNA replication and transcription

  • Prof. Julio Montoya
• 37 min
  6. Mitochondrial production of reactive oxygen species 1

  • Prof. Martin Brand
• 22 min
  7. Mitochondrial production of reactive oxygen species 2

  • Prof. Martin Brand
• 35 min
  8. Mitophagy - the degradation of dysfunctional organelles

  • Dr. Anna Vainshtein
• Mitochondrial Biogenesis in Physiological Conditions
• 21 min
  9. Thyroid hormone and mitochondrial biogenesis

  • Dr. Joachim Weitzel
• 28 min
  10. Exercise-induced mitochondrial biogenesis in muscle 1

  • Prof. David Hood
• 37 min
  11. Exercise-induced mitochondrial biogenesis in muscle 2

  • Prof. David Hood
• Mitochondrial Biogenesis in Aging and Disease
• 33 min
  12. Mitochondrial diseases: an update

  • Dr. Ayesha Saleem
• 59 min
  13. Exercise and nutrition in mitochondrial disorders

  • Prof. Mark Tarnopolsky
• 42 min
  14. Mitochondrial disorders and neurodegeneration

  • Dr. Jan-Willem Taanman
• 33 min
  15. Reprogramming oxidative phosphorylation in cancer

  • Prof. José Cuezva
• 44 min
  16. Possible roles of mitochondrial biogenesis in aging

  • Dr. Aubrey de Grey
• 31 min
  17. Mitochondria in reproduction and fertility: mitochondria and gametes 1

  • Prof. Pascale May Panloup
• 30 min
  18. Mitochondria in reproduction and fertility: mitochondria and embryo 2

  • Prof. Pascale May Panloup
• 35 min
  19. Mitochondrial morphology and ultrastructure in skeletal muscle

  • Prof. Martin Picard
• 19 min
  20. Improving mitochondrial phenotypes with pharmaceuticals

  • Prof. Christopher Perry
• Mitochondria, Cell Signaling and Apoptosis
• 42 min
  21. Mitochondrial permeability transition

  • Prof. John Lemasters
• 47 min
  22. Pathways of apoptosis in muscle 1

  • Prof. Stephen E. Alway
• 42 min
  23. Pathways of apoptosis in muscle 2

  • Prof. Stephen E. Alway
• 21 min
  24. Controlling mitochondrial reactive oxygen species production for cell signaling 1

  • Dr. Ryan J. Mailloux
• 32 min
  25. Controlling mitochondrial reactive oxygen species production for cell signaling 2

  • Dr. Ryan J. Mailloux
• Archived Lectures *These may not cover the latest advances in the field
• 44 min
  26. Reactive oxygen species and myocardial apoptosis

  • Dr. Zhi-Qing Zhao
• 34 min
  27. Mitochondrial biogenesis as a result of exercise

  • Dr. Darrell Neufer
• 30 min
  28. Mechanisms of mitochondrial fusion and fission

  • Dr. Luca Scorrano
• 30 min
  29. Mitochondrial heterogeneity in cells

  • Dr. Tony Collins
• 40 min
  30. Mitochondrial diseases: an update

  • Prof. Salvatore DiMauro
• 41 min
  31. Calcium signaling and mitochondrial function

  • Prof. David Nicholls
• 35 min
  32. Mitochondrial biogenesis during gametogenesis and embryonic development

  • Prof. Pascal Reynier
• 33 min
  33. Mitochondrial complex assembly

  • Dr. Leo Nijtmans
• 33 min
  34. Evolutionary and developmental variation in muscle mitochondrial content

  • Prof. Christopher Moyes
• 33 min
  35. Muscle mitochondrial function and biogenesis with aging

  • Dr. Russell Hepple
• 23 min
  36. The mitochondrial pathway in apoptosis

  • Prof. Douglas Green
• 40 min
  37. Nuclear control of respiratory chain expression by transcriptional activators and coactivators

  • Prof. Richard Scarpulla
• 38 min
  38. Mitochondrial disorders and neurodegeneration

  • Prof. Anthony Schapira
• 43 min
  39. Respiration, reactive oxygen species and uncoupling proteins

  • Prof. Martin Brand

Printable Handouts

PDF

Navigable Slide Index

1. Introduction
2. Oxphos complex assembly
3. Outline: introduction and OXPHOS assembly
4. Outline: complexes IV, I and supercomplexes
5. Mitochondria: oval shaped organelles
6. Two membranes, 4 compartments
7. Mitochondria form a dynamic network
8. Mitochondria contain extra chromosomal DNA
9. Mitochondrial DNA
10. Main function of mitochondria: ATP production
11. Phosphorylation, e- transport and H+ generation
12. All components form together a functional complex
13. What are the OXPHOS complex components?
14. Nuclear and mitochondrial subunits
15. Co-evolution of mitochondrial and nuclear subunits
16. Additional components
17. How is the assembly coordinated
18. MtDNA replication, translation and regulation (1)
19. Over-expressing a single subunit (Cox4p)
20. Assembly
21. Which tools are required and what can go wrong?
22. MtDNA replication, translation and regulation (2)
23. Why study OXPHOS complex assembly?
24. How do we study assembly?
25. Two-dimensional blue-native PAGE
26. Complex IV (cytochrome c oxidase)
27. Cytochrome c oxidase assembly intermediates
28. Putative COX assembly pathway
29. The COX assembly is distributed in patients
30. Complex IV assembly
31. Complex I
32. Complex I assembly
33. Is complex I assembly a sequential process?
34. Is complex I a semi-sequential process?
35. Subunits and topology (1)
36. Complex I assembly in N. crassa
37. The modular evolution model
38. Conditional complex I assembly system creation
39. Dynamics of complex I subcomplexes
40. Composition of subcomplexes (1)
41. Composition of subcomplexes (2)
42. Subcomplexes of complex I
43. Complex I subunits presence per subcomplexes
44. Model for human complex I assembly
45. Subunits and topology (2)
46. Complex I crystal structure
47. Human complex I assembly
48. Complex I assembly distribution: deficient patients
49. Disturbed CI assembly in CI deficient patients
50. CI assembly proteins
51. Complex I chaperone NDUFAF1
52. Is NDUFAF1 localized in the mitochondrion?
53. Is NDUFAF1 involved in complex I assembly?
54. Involvement of assembly chaperones?
55. NDUFAF1 is a genuine CI assembly chaperone
56. Super complexes
57. Tracing the complexes on a gel
58. Respiratory complexes organised as respirosome
59. Some implications of supercomplexes
60. OXPHOS supercomplexes
61. The role of supercomplexes in cristae morphology
62. Supercomplexes: summary
63. Concluding remarks
64. Acknowledgments

Topics Covered

• The mitochondrial oxidative phosphorylation system
• Components: mitochondrial subunits, nuclear subunits and prosthetic groups
• Coordination of assembly
• Factors involved in assembly
• Defects in OXPHOS assembly
• Complex IV assembly
• Complex I assembly
• Complex I assembly defects
• Chaperones for complex I
• Supercomplexes of the OXPHOS system

Links

Series:

• Mitochondrial Biogenesis

Categories:

• Biochemistry
• Cell Biology

Talk Citation

Publication History

• Published on October 1, 2007
• Archived on October 28, 2018

Financial Disclosures

• Dr. Leo Nijtmans has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.