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Printable Handouts
Navigable Slide Index
- Introduction
- Why monitor development of drug resistance?
- Diagnosis and monitoring: different disciplines
- Methods for determining resistance
- Molecular tools detect genotypic variation
- The tools to detect genotypic variation
- Considerations in adopting any diagnostic test
- Rapid techniques in clinical practice
- Streptococcus pneumoniae
- Penicillin resistance in S. pneumoniae
- Resistance diagnosis in S. pneumoniae
- S. pneumoniae: improve on culture
- S. pneumoniae: species specific PCR
- S. pneumoniae: MIC vs. RFLP pattern
- S. pneumoniae: MIC vs. RFLP pattern - results
- Mycobacterium tuberculosis
- Resistance diagnosis in M. Tuberculosis (1)
- Distribution of mutations in the RpoB gene
- M. tuberculosis resistance determination
- INNO-LiPA line probe assay
- London isoniazid resistant outbreak strains
- LiPA results for outbreak samples
- RifR samples from the north London outbreak
- Limitations
- Resistance diagnosis in M. Tuberculosis (2)
- Resistance diagnosis in M. Tuberculosis (3)
- Methicillin resistant Staphylococcus aureus
- Resistance diagnosis in MRSA (1)
- Resistance diagnosis in MRSA (2)
- BD GeneOhm MRSA assay
- Molecular target: MRSA DNA
- BD GeneOhmTM MRSA assay test procedure
- How is this screen being used?
- The culture step - do we need it?
- Developing technologies
- Molecular diagnosis of resistance
Topics Covered
- Diagnosis and monitoring are different disciplines
- Molecular tools detect genotypic variation
- Role for molecular tools in clinical practice
- Examples are penicillin binding proteins in Streptococcus pneumoniae, drug resistant Mycobacterium tuberculosis and MRSA
Links
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Talk Citation
McHugh, T. (2009, December 31). Molecular diagnosis of antibiotic resistance [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved December 3, 2024, from https://doi.org/10.69645/KLNN8214.Export Citation (RIS)
Publication History
Financial Disclosures
- Dr. Tim McHugh has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.